Antibacterial activity of purified esterase from Lactobacillus gasseri against Pseudomonas aeruginosa causing wound infections | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 22 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.430079.3292 | ||
| Authors | ||
| Inas Sattar Abd* 1; Halah Farazdaq1; Asmaa Sattar Abd2 | ||
| 1Collage of Science, Al-karkh University of science, Baghdad, Iraq | ||
| 2Badgdad AL-Karkh Health Directorate, FETP Division, Baghdad, Iraq | ||
| Abstract | ||
| Background/aim: Human opportunistic pathogen Pseudomonas aeruginosa is frequently found, linked to nosocomial infections such as burns and wound infections at the surgery site, and continues to be a serious health risk, particularly for severely ill and immunocompromised individuals. Thus, the aim of this study to detect the activity of purified esterase from Lactobacillus gasseri as antibacterial agent against MDR P. aeruginosa causing wound infections. Methods: Here, we screened the different Lactobacillus species for esterase synthesis and subsequently purified the esterase using chromatographic techniques.The minimum inhibitory concentration (MIC) of the purified esterase in microtiter plates was ascertained by utilizing a broth dilution technique of analysis for detecting the antimicrobial activity of esterase. Results: It was discovered that Lactobacillus gasseri was the most potent producer for esterase compared to Lactobacillus fermentum. Nine strains of P. aeruginosa, an opportunistic bacterium, were obtained from wound infections. The MIC values with the range from 4 to 64 µg/ml, the purified esterase showed a higher level of inhibition for Pseudomonas aeruginosa isolates than the most widely used antibiotics, such as ciprofloxacin and gentamicin. Conclusion: Thus, an esterase enzyme shows promise as a bioactive substance that inhibits the growth of bacteria in order to combat hazardous invasive Pseudomonas aeruginosa infections. | ||
| Keywords | ||
| P. aeruginosa; esterase; wound infections | ||
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