PARASITOLOGICAL AND MOLECULAR DETECTION OF TRYPANOSOMA SPECIES IN CATTLE IN NINEVEH GOVERNORATE, IRAQ | ||
| Assiut Veterinary Medical Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 23 October 2025 PDF (538.48 K) | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.2025.372891.1652 | ||
| Authors | ||
| WAAD KHALID KHALAF* ; WASAN AMJAD ALOBAIDII | ||
| Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq. | ||
| Abstract | ||
| Data about bovine trypanosomiasis and its causes in Nineveh Governorate and Iraq are limited. In relation to the origin of cattle, trypanosomes have never been discovered before through molecular study. Blood samples from 221 cattle were examined by microscopy and conventional polymerase chain reaction (PCR) assays in different areas of Nineveh Governorate, Iraq, from October 2023 to August 2024. The Sanger sequencing method was used to sequence the PCR product of the positive samples, which were delivered to Psomagen in the USA. The study detected Trypanosoma in 12.22% by microscopy and 20.81% by using conventional PCR, with significant increases in the PCR assay. Regarding the risk factors for animals (gender, age, and breed) from Positive samples of the PCR assay, significant increases at the level (P<0.05) were recorded in female groups 31.34%, age (>2 years) 25.52% and imported breed 26.83%. Trypanosoma vivax was the predominant species in the study area. The genetic sequencing findings were saved as text files in the FASTA format. Selected strains were submitted to NCBI's GenBank for registration and the acquisition of unique accession numbers, LC830952.1 for T. congolense, LC830948.1 for T. vivax and LC830954.1 for T. brucei. The percentage of similarity to the nucleotide sequence of the strains that were diagnosed in the study, when BLAST was performed in the database, compared to the nucleotide sequences of the international strains, which were identified during this study and which were used for the purpose of constructing the genetic phylogenetic tree. The results showed that there was a variation in similarity rates between the local isolates and other strains, which was limited to countries on the African continent. | ||
| Keywords | ||
| Trypanosoma; Cattle; Microscopy; Molecular; Phylogeny; Iraq | ||
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