Protein kinases: activity and inhibition by insecticides and natural products | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 6, Issue 1, June 1998, Pages 35-48 PDF (851.4 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.1998.461430 | ||
| Author | ||
| Mahmoud M. Abo-El-Saad* | ||
| Pesticide Chemistry Dept, Faculty of Agriculture, El-Shatby, Alexandria University | ||
| Abstract | ||
| Calcium-phospholipid-dependent protein kinase (PKC), polypeptide-dependent protein kinase (PKP) and epidermal growth factor-receptor (EGF-R) as a tyrosine kinase were prepared from rat rain, yeast, and Ad31_ human epidermoid carcinoma eels, respectively. Their Phosphorylation activities and inhibition assays were carried out radiographically by two independent methods, filter paper assay and autoradiogram of polyacrylamide gel electrophoresis. The autoradiogram of the A431 tyrosine Kinase activity showed. its ability to phosphorylate itself of approximately 170 KD. Mammalian hormone epidermal growth factor (EGF) showed that A431 tyrosine kinase at the same band (170 KD) was highly stimulated by EGF. On the contrary, the autophosphorylation of this tyrosine kinase was inhibited by over 50% at 10 uM gossypol concentration, where as in the presence of EGF, the inhibition was only 32% at the same concentration of gossypol. Moreover, several chlorinated hydrocarbons compounds, DDT, endrin, dieldrin, aldrin and chlordane and deltamethrin from synthetic pyrethroids, all showed inhibitory effects ofthe activity of PKP. Staurosporine (microbial chemical product), aldrin, DDT, and deltamethrin showed strong inhibitory effects of the activity of PKC, The results were discussed in view of mammalian toxicity of these compounds and their Selectivity as insecticides | ||
|
Statistics Article View: 2 PDF Download: 1 |
||
