Insecticide resistance testing in the cotton pink bollworm | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 3, Issue 1, June 1991, Pages 1-12 PDF (523.2 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.1991.461544 | ||
| Authors | ||
| Osman A. El Gougary* 1; L. P. Schouest2 | ||
| 1Plant Protection Institute. El Sabahia, Alexandria, Egypt. | ||
| 2Department of Entomology, University of California, Riverside, California 9252 1 USA | ||
| Abstract | ||
| LC50 values for third instar pink bollworm were obtained for laboratory susceptible and field strains (Palo Verde Valley, CA) for the following compounds, permethrin. Chlorpyrifos, malathion, methomyl, chlordimeform, S,S,S-tributyl phosphorotrithioate (DEF), piperonyl butoxide (PBO)), and Bacillus thuringiensis subsp. toxin. Mortality after topical applications was recorded at 24 and 48 hrs. LC50 values were also obtained for selected Insecticide/ synergist combinations. Resistance ratios were calculated for each insecticide and Insecticide/ synergist combinations. The synergistic ratio was calculated for each combination for field arid laboratory populations. At the same time, determinations of total protein and measurements of esterase activity (as naphthyl acetate) were also obtained in effort to correlate esterase activity to the presence of resistance in the field strain. Significant resistance was found in the field to permethrin and methomyl. Permethrin was also found to have a high synergistic ratio, suggesting a possible metabolic resistance component. The field strain was found to have significantly higher esterase activity when compared to the laboratory strain. Though higher total protein levels were found in the field strain. Some unknown percentage of resistance (possibly metabolic) can be attributed to elevated esterase levels, especially for resistance to permethrin. | ||
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