| Microbial Remediation: Harnessing Pseudomonas to Tackle Sodium Dodecyl Sulphate Polluted Waters | ||
| Egyptian Journal of Medical Microbiology | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 July 2026 PDF (567.54 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.426296.1884 | ||
| Authors | ||
| Safaa A. Al-Obaidi* 1; Tamara H. Zedan2; Abdulameer M. Ghareeb1 | ||
| 1Institute of Genetic Engineering and Biotechnology for Post Studies, University of Baghdad, Baghdad, Iraq | ||
| 2College of Biotechnology, Al-Nahrain University, Baghdad, Iraq | ||
| Abstract | ||
| Background: Sodium dodecyl sulphate (SDS) is an ionic surfactant that widely use in daily detergent and cleaning products. It one of the abundance water contaminants. This paper focus on SDS bioremediation using Pseudomonas aeruginosa as a sole bacterial species compared with microbial consortium. Methodology: Four this purpose, two continuous bioreactors were used to degrade 1gl-1 SDS as the sole carbon source. Pseudomonas aeruginosa was isolated from peaty wastewater soil and it was identified by VITEK 2 and 16S rRNA. Nevertheless, in bioreactor A, Pseudomonas aeruginosa was used as a pure bacterial culture while in bioreactor B, a peaty soi contains an indigenous microbial community includes Pseudomonas aeruginosa and other synergetic microbial community. Results: The concertation of SDS was declined to zero in bioreactor B compared with 0.18 gl-1 in bioreactor A. GC-MS was used to elucidate the SDS degradation products in the effluent of both bioreactors. Decanoic acid and dodecanal were the main SDS degradation products the effluent of bioreactor A while, a complete mineralization was determined in the effluent of bioreactor B. Scan electron microscope (SEM) magnification image highlighted that Pseudomonas aeruginosa was effectively growth and immobilized on the bio-ball surface that used in bioreactor A which contributed in SDS bioretention. Conclusion: the conducted experiments finding confirmed that microbial community contains Pseudomonas aeruginosa remediated SDS more efficient than Pseudomonas aeruginosa pure culture. | ||
| Keywords | ||
| Bioreactor; bioremediation; Sodium dodecyl sulphate; Pseudomonas aeruginosa; peaty soil | ||
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