Cryopreserved Semen of Holstein and Buffalo Bulls was Adversely Affected by High Pomegranate Juice Levels | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 26 October 2025 PDF (858.19 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.390960.2882 | ||
| Authors | ||
| Nibras Ali Alsulayih1; Mohammed Ali Abdelghani* 2; Sobhy Farag3; El-Shenawy El-Seify4; Abd El-Salam Metwally3; Atef Salem3; Razan Naji5; Mohamed Elsharawy6 | ||
| 1postgradute | ||
| 2colleges of Veterinary medicine | ||
| 3Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt | ||
| 4Animal Production Research Institute; Agriculture Research Center, Ministry of Agriculture, Dokki, Giza, Egypt | ||
| 5Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, AL-Ahsa, 31982, Saudi Arabia | ||
| 6Faculty of Agriculture, Kafrelsheikh UnDepartment of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egyptiversity | ||
| Abstract | ||
| This study investigated whether pomegranate juice (PJ) ameliorates cryopreserved semen in Holstein and buffalo bulls. Control extenders and varying concentrations of PJ (1%, 2%, 4%, 6%, and 8%) were utilized to dilute, equilibrate, and cryopreserve semen. Results demonstrated that after dilution, sperm motility and viability percentages at 6% and 8% were lower (P < 0.05) than for controls in Holstein bulls. However, in buffalo bulls, percentages of motile and viable sperm did not differ (P > 0.05) among controls and variant PJ concentrations. After equilibration in Holstein bulls, percentages of sperm motility and viability at 1% and control were higher (P < 0.05) than those at 8%. In buffalo bulls, percentages of sperm motility were higher at 2% (P < 0.05) than at 6% and 8%. In buffalo bulls, percentages of sperm motility at 2% were higher than at 8%, and percentages of sperm viability at 1% were higher (P < 0.05) than at 8%. In buffalo bulls, progressive sperm motility at 6% was higher than in control and 1%. In Holstein bulls at 8%, DAP, DCL, DSL, VAP, VCL, and BCF were lower (P < 0.05) than at 1% and control. In buffalo bulls, DAP, DCL, DSL, VAP, and VCL were lower at 1% than at 4% and 6%. In conclusion, in buffalo bulls, low doses of PJ improved sperm kinetics, but in Holstein bulls, PJ had opposite effect; nevertheless, sperm kinetics and features were negatively impacted by high concentrations. | ||
| Keywords | ||
| Bulls; semen; pomegranate juice; cryopreservation | ||
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