Spexin Protects Against Oxidative Stress-Induced Apoptosis of Sciatic Nerve Among Rats with Diabetic Peripheral Neuropathy | ||
| Zagazig University Medical Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 26 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zumj.2025.422866.4182 | ||
| Authors | ||
| Hadeel Elsherbini* 1; Basma .S. Elsayed2; Sara G. Seada3; Sherein F. El-sayed4 | ||
| 1Lecturer of physiology, Faculty of medicine, Zagazig university | ||
| 2Lecturer of Medical Biochemistry, Faculty of medicine, Zagazig university | ||
| 3Lecturer of Physiology, Faculty of medicine, Zagazig university | ||
| 4Assistant Professor of Medical Physiology, Medical physiology department, Faculty of Medicine, Zagazig University | ||
| Abstract | ||
| Background: Diabetic peripheral neuropathy (DPN) is considered among the most common consequences of diabetes mellitus (DM). Spexin-14 amino acid peptide hormone- is expressed in brain and peripheral tissues. It plays an increasing importance in metabolic illnesses because of its contribution in energy homeostasis and food intake besides its anti-inflammatory, anti-oxidative, properties. This work aimed to investigate the possible ameliorative impact of spexin towards the development of DPN in type-2 DM model induced experimentally in rats. Methods: 24 male healthy albino rats, with body weights 160-180 gm, were categorized into 3 groups: group I control group. Group II (induced type II Diabetic group) included rats fed a high-fat fructose diet for 8 weeks then a single dose of streptozotocin (35 mg/kg BW, intraperitoneal). And Group III: DM + Spexin group (35 μg/kg/day). Tail cold allodynia test, Tail flick test, Rota-rod test and Gait test (behavioral tests) were done. Glucose, insulin, lipid profile, inflammatory and oxidative stress mediators serum values were measured. Serum TNF-α and IL-1 were assessed by ELISA. Gene expression of BCL2, and Caspase-3 concentrations in isolated sciatic nerve were measured by RT PCR Analysis finally histopathological analysis. Results: Giving Spexin to diabetic rats ameliorated deterioration in neuropathic behavior tests, reduced serum levels of glucose, improvement in lipid profiles and insulin resistance with a significant restoration of antioxidant enzyme levels, IL1, and TNF. Furthermore, a significantly elevated expression of Bcl-2 and declined Caspase-3 gene expression. Conclusion: SPX improves diabetes induced peripheral neuropathy and has hypoglycemic, hypolipidemic, antioxidant, anti-inflammatory properties. | ||
| Keywords | ||
| Spexin; Peripheral Neuropathy; Type-2 Diabetes; Rat | ||
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