Inhibition of spermatogenesis and DNA-synthesis in male gonads of Pectinophora gossypiella (Saunders) in response to the development of cyanophos-resistance
El-Sorady, A., Rofail, M., Massoud, M., Rashad, A., Khaled, A. (1999). Inhibition of spermatogenesis and DNA-synthesis in male gonads of Pectinophora gossypiella (Saunders) in response to the development of cyanophos-resistance. EKB Journal Management System, 7(2), 1-18. doi: 10.21608/jpces.1999.462099
A. E. M. El-Sorady; Mona F. Rofail; M. M. Massoud; Amira M. Rashad; A. E. Khaled. "Inhibition of spermatogenesis and DNA-synthesis in male gonads of Pectinophora gossypiella (Saunders) in response to the development of cyanophos-resistance". EKB Journal Management System, 7, 2, 1999, 1-18. doi: 10.21608/jpces.1999.462099
El-Sorady, A., Rofail, M., Massoud, M., Rashad, A., Khaled, A. (1999). 'Inhibition of spermatogenesis and DNA-synthesis in male gonads of Pectinophora gossypiella (Saunders) in response to the development of cyanophos-resistance', EKB Journal Management System, 7(2), pp. 1-18. doi: 10.21608/jpces.1999.462099
El-Sorady, A., Rofail, M., Massoud, M., Rashad, A., Khaled, A. Inhibition of spermatogenesis and DNA-synthesis in male gonads of Pectinophora gossypiella (Saunders) in response to the development of cyanophos-resistance. EKB Journal Management System, 1999; 7(2): 1-18. doi: 10.21608/jpces.1999.462099

Inhibition of spermatogenesis and DNA-synthesis in male gonads of Pectinophora gossypiella (Saunders) in response to the development of cyanophos-resistance

Journal of Pest Control and Environmental Sciences
Volume 7, Issue 2, June 1999, Pages 1-18    PDF (9.27 M)
Document Type: Original Article
DOI: 10.21608/jpces.1999.462099
Authors
A. E. M. El-Sorady* 1; Mona F. Rofail1; M. M. Massoud2; Amira M. Rashad1; A. E. Khaled3
1Plant Protection Research Institute, ARC, Dokki, Egypt.
2Plant Protection Dept., Faculty of Agric., Saba Basha, Alex. Univ., Egypt.
3Agricultural Botany Dept., Faculty of Agric., Saba Basha, Alex. Univ., Egypt.
Abstract
Selection of newly-hatched larvae of the pink bollworm Pectinophora gossypiella (Saunders) (Lep., Gelechiidae), field strain reared in laboratory for several generations free from insecticidal pressure, with Cyanophos for 32 generations, besides three relaxed generations (G33-G35) was carried out. The effect of development of Cyanophos-resistant, relaxed and susceptible strains on both male gonad relative nuclear DNA-content and the proliferation of differential stages of spermatogenesis was studied. The obtained results showed that Cyanophos, as a selective agent, possesses a high resistance potential to the pink bollworm. The selection indicated a considerable degree of homogeneity towards the development of Cyanophos resistance and achieved 42.76 fold at the 32and generation. While the third relaxed generation (G35) showed the highest slope of 3.1 accompanied by high decrease in resistance (27.80 fold) leading to rapid return to resistance reversion, nearly equals to G20 to be susceptible again. Moreover, the achieved results showed that the development of Cyanophos resistance increased the percentages of male gonad cells having 2C-DNA-content and the abnormal ones having more than 4C-DNA-content. Classifying the differential stages of spermatogenesis indicated that the proportions of stem cells, spermatogonia A & B and spermatocytes were the highest in the resistant strain. On the other hand, the development of Cyanophos-resistance induced the least percentages of cells having 1C-, 3C- and 4C-DNA-content and associated with the decrease of young & mature spermatids, immature & mature spermatozoa, proleptotene, pachytene and certain cell division stages (diakinesis, G2 and metaphase I).
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