Effect of lead exposure on the level of protein kinase c isolated from mouse brain | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 7, Issue 3, December 1999, Pages 51-68 PDF (5.66 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.1999.462118 | ||
| Authors | ||
| Mahmoud M. Abo-El-Saad* 1; Samia A. Abo-Seada2; Amina T. Farag2 | ||
| 1Faculty of Agriculture, Pesticide Chemistry & Toxicology Department, Alexandria University. | ||
| 2Plant Protection Research Institute, Agriculture Research Center2, Alexandria, Egypt. | ||
| Abstract | ||
| Subchronic exposure of mice to different concentrations of lead has been performed for 70 days. F1-pups were produced from different cross-mating animals of F0 female and male mice, which have been treated by lead acetate at different concentrations of 0.0, 26, 260 and 2600 ppm. The level of protein kinase C (PKC) in brains of treated and untreated animals was determined by using immunoblot analysis on polyvinylidene difluoride (PVDF) membrane against anti-protein kinase C antibody. Immunoblot analysis showed that the level of PKC was significantly decreased in most of the treated animals either at F0 premated females or males. At 260 and 2600 ppm, the reduction level of PKC was about 16.7 and 100% for females and 33.7 and 50% for males respectively. During mating, periods, the reduction level of this enzyme isolated from females after gestation and lactation was observed in all the exposed females and it was 100% by 2600 ppm. Furthermore, PKC level of female pups produced from treated females mated with treated or untreated males, was significantly decreased compared to those pups produced from untreated animals. There are three major reasons for the reduction of PKC which's are could be concluded from the present data (a) Dramatic reduction which occurred in treated F0 male and female mice during gestation and lactation period. (b) The level of PKC from F0 female after gestation and lactation and male mice after mating, was apparently reduced and (c) severe reduction in the level of this enzyme was observed in F1-female mice produced from treated animals. | ||
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