The impact of chitin isolation process sequences on the physicochemical properties and antimicrobial activity of the resultant chitosan compounds | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 20, Issue 1, 2012, Pages 49-60 PDF (980.25 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.2012.462316 | ||
| Authors | ||
| Yahya I. Kaseem; Hanay A. Assar; Sayed A. M. Abdallah* | ||
| Department of Pesticide Chemistry and Technology, Faculty of Agriculture, 21545-Elshatby, Alexandria University, Alexandria, Egypt | ||
| Abstract | ||
| Three chitosan (CS) compounds were prepared by deacetylation (DA) of chitin isolated from shrimp shell using different sequences of isolation process steps which include deproteinization (DP), demineralization (DM) and decolorization (DC). The chitosan compounds produced were DP MCA, DMPCA and DCMPA (the letter sequences indicate the sequential process used to prepare chitosan). The impact of alternate steps sequence through chitin isolation process on the physicochemical properties and biological activity of resultant chitosan, compared with a commercial chitosan compound was investigated. The results indicated that the differences in the solubility of the three chitosan compounds in 1 % acetic acid. Where the isolation process started with DC step, a chitosan with high average viscosity molecular weight of 4.26 ×105 Da and high degree of deacetylation (DDA) 87.8 % was obtained compared with DMPCA and DPMCA. The antibacterial assessment of resultant chitosan compounds was performed against Corynebacterium spp. and Erwinia amylovora and the results are expressed as a minimum inhibitory concentration (MIC). In general, the results indicated that all chitosan compounds have a good antibacterial activity. However, the bacterium of Corynebacterium spp was more sensitive to chitosan compounds than E. amylovora where the MIC in average was 650 µg mL-1 and 2150 µg mL-1 respectively. The antifungal assessment was also performed against fungi of Fusarium culmorium, Aspergillus niger and Rizoctonia solani. The results are expressed as an effective concentration that inhibits 50 % of mycelia growth (EC50). The result demonstrated a good and closer antifungal activity for the three chitosan compounds, however, DCMPA chitosan has low antifungal activity compared with other two chitosan compounds with EC50 values ranged between 2339 to 3430 µg mL-1 for all tested chitosan compounds. | ||
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