The potency of the vegetative insecticidal protein (Vip3A), in comparison with crystalline (Cry) toxins of Bacillus thuringiensis against Spodoptera littoralis (Boisd.)
Khalafallah, M., El-Shahawi, F., Sherby, S., Mansour, N. (2012). The potency of the vegetative insecticidal protein (Vip3A), in comparison with crystalline (Cry) toxins of Bacillus thuringiensis against Spodoptera littoralis (Boisd.). EKB Journal Management System, 20(1), 61-78. doi: 10.21608/jpces.2012.462317
M. H. Khalafallah; F. I. El-Shahawi; S. M. Sherby; N. A. Mansour. "The potency of the vegetative insecticidal protein (Vip3A), in comparison with crystalline (Cry) toxins of Bacillus thuringiensis against Spodoptera littoralis (Boisd.)". EKB Journal Management System, 20, 1, 2012, 61-78. doi: 10.21608/jpces.2012.462317
Khalafallah, M., El-Shahawi, F., Sherby, S., Mansour, N. (2012). 'The potency of the vegetative insecticidal protein (Vip3A), in comparison with crystalline (Cry) toxins of Bacillus thuringiensis against Spodoptera littoralis (Boisd.)', EKB Journal Management System, 20(1), pp. 61-78. doi: 10.21608/jpces.2012.462317
Khalafallah, M., El-Shahawi, F., Sherby, S., Mansour, N. The potency of the vegetative insecticidal protein (Vip3A), in comparison with crystalline (Cry) toxins of Bacillus thuringiensis against Spodoptera littoralis (Boisd.). EKB Journal Management System, 2012; 20(1): 61-78. doi: 10.21608/jpces.2012.462317

The potency of the vegetative insecticidal protein (Vip3A), in comparison with crystalline (Cry) toxins of Bacillus thuringiensis against Spodoptera littoralis (Boisd.)

Journal of Pest Control and Environmental Sciences
Volume 20, Issue 1, 2012, Pages 61-78    PDF (1.5 M)
Document Type: Original Article
DOI: 10.21608/jpces.2012.462317
Authors
M. H. Khalafallah; F. I. El-Shahawi; S. M. Sherby; N. A. Mansour*
Pesticide Chemistry and Technology Dept., Fac. of Agric., Alexandria University, El-Shaby, Alexandria, Egypt.
Abstract
The susceptibility measurements were performed on larvae of the cotton leafworm, Spodoptera littoralis with two Bacillus thuringiensis authentic strains; Bt subsp. kurstaki (HD-1) and Bt subsp. entomocidus (60.5). The protoxin (≈130 kDa), the active toxin (≈ 66 kDa) in the cell pellet after sonication and the vegetative insecticidal protein (≈ 88 kDa) were confirmed by SDS-PAGE. The vegetative insecticidal protein (Vip3A) can be detected in the growth media. LC50 values were compared with respect to the LC-p lines statistical parameters (slope & Chi2). All parameters were determined at different exposure periods. In general, the HD-1-Vip3A toxin is more toxic against Spodoptera larval instars than the tested Cry toxins (entomocidus (60.5) and kuristaki HD-1). The toxicity is dependent on the larval instar age.
Statistics
Article View: 2