ATPase natural inhibitor, purification, and its interaction with pesticide effect | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 1, Issue 1, 1987, Pages 1-6 PDF (1.18 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.1987.462341 | ||
| Authors | ||
| M. Massoud* ; M. Abdel-Ghany; Nader Shaker | ||
| Pesticide Chemistry Dept. College of Agriculture, University of Alexandria, Alex. Egypt. | ||
| Abstract | ||
| The natural ATPase inhibitor was extracted from both honey bee thorax and beef heart by exposing the purified ATPase enzyme to heat treatment followed by centrifugation and purification of the supernatant by using DEAE-Sephadex chromatography. The molecular weight of honey bee ATPase natural inhibitor was calculated as 8000 dalton and appears in one protein band using sodium dodecylsulfate-slab polyacrylamide gel electrophoresis (SDS-PAGE). The inhibitory effect of the extracted crude and purified natural ATPase inhibitors were studied against their F1 or mitochondrial ATPase. The interaction of natural inhibitor with ATPase activity was compared kinetically with gossypol (natural botanical material) and dicofol (pesticide). Natural ATPase inhibitor showed uncompetitive interaction with ATP substrate which differed from the recorded noncompetitive effect of gossypol and dicofol on ATPase activity. The interaction between natural ATPase inhibitor in presence of gossypol or dicofol on ATPase activity was studied. | ||
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