ATPase natural inhibitor, purification and its interaction with pesticide effect | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 1, Issue 1, 1987, Pages 1-6 PDF (799.38 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.1987.462341 | ||
| Authors | ||
| M. Massoud* ; M. Abdel-Ghany; Nader Shaker | ||
| Pesticide Chemistry Dept. College of Agriculture, University of Alexandria, Alex. Egypt. | ||
| Abstract | ||
| The natura-l ATP ase inhibitor was extracted from processes but has a little effect on ATP svnthesis. both honey bee thorax and beef heart by exposing the Natural inhibitor is an essential component of the purified AT Pase enzyme to heat treatment followed by system catalyzing oxidative phosphorylation. The molecular weight of honey bee .ATPase natural characteristic role to interact with other natural inhibitor was calculated as 8000 da.lton and appears materials (gossypol) or pesticide (dicofol) on mitc in one protein band using sodium dodecylsulfate-slab chondrial-so.luble A TP ase activity. polyacrylamide gel electrophoresis(SDS-PAGE). The inhibitory effect of the extracted crude and purified natural ATPase inhibitors were studied against their F or mitochondrial ATPase. The interaction of natural inhibitor with ATP ase activity was compared kinetically with gossypol (natural botenical material) and dicofol (pesticide). Natural ATPase showed uncompetitive interaction with ATP substrate shwed differed from the recorded noncompetitive effec of gossypol and dicofol on ATPase activity. The interaction between natural ATP ase inhibitor in presence of gossypol or dicofol on ATPase activity was studied. | ||
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