Optimization of cyanophycin granule polypeptide production of some isolated cyanobacterial species | ||
| Mansoura Journal of Biology | ||
| Volume 47, Issue 2, June 2020, Pages 35-47 PDF (1.39 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2020.462396 | ||
| Authors | ||
| Shimaa M. Ragab* ; Ghada Samir Abo-Elwafa; Mohammad I. Abdel-Hamid | ||
| Botany Department, Faculty of Science, University of Mansoura, Egypt | ||
| Abstract | ||
| This paper aimed primarily at investigating the potential of cyanophycin production of some selected cyanoprokaryotes. For this purpose, nine different cyanobacteria species namely. Oscillatoria obscura, Anabaena anomala (isolate 1), Anabaena anomala (isolate 2), Anabaena oryzae, Anabaena khannae, Nostoc kihlmani, Nostoc sp, Synechococcus sp, Spirulina platensis, were selected as test species. The cyanobacteria species were grown in standard BG11 nutrient medium and incubated for 30 days under favorable growth condition at 25±2oC and with continuous light of 2.789w-2. Different test cyanobacteria exhibited wide variations in both biomass (0.25 - 0.8 freeze-dry wt g l-1) and cyanophycin content (0.03-0.224g l-1 ). The experimental results indicated that the cyanobacterium Anabaena anomala (isolate 2) maintained relatively higher biomass production and CGP content. Therefore, this cyanobacterium was selected for further screening growth experimental design to define the best growth medium composition (mgl-1). Compared to control, the results indicated that the modified composition of BG11 (2250mg NaNO3, 60mg K2HPO4, 112.5mg MgSO4.7H2O, 54mg CaCl2.2H2O, 3.0mg citric acid, 9mg ferric ammonium citrate, 1.5mg EDTA, 30mg Na2CO3 , 0.031mg H3BO3, 0.255mg MnSO4.H2O, 0.143mg ZnSO4.7H2O, 0.0038mg CuSO4.5H2O, 0.0063mg (NH4)6Mo7O24.4H2O, 25ml Soil solution, 500mg NaCl) increased the biomass production by 86.25% (from 0.8gl-1 to 1.49gl-1) and cyanophycin content by 96.43% (from0.224gl-1to0.44gl-1).Based on these results it has become evident that modified BG11medium composition induced highly significant( p ≤ 0.01) increase in both biomass production and CGP content of cyanobacterium Anabaena anomala (isolate 2) and open new research window for further large –scale growth experiments. | ||
| Keywords | ||
| cyanobacteria; cyanophycin granule polypeptide (CGP); Plackett–Burman; Anabaena anomala | ||
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