Biodegradation of phenol by Pseudomonas putida strain B1isolated from Egyptian soil | ||
| Mansoura Journal of Biology | ||
| Volume 46, Issue 1, March 2020, Pages 46-51 PDF (791.01 K) | ||
| Document Type: Research Article | ||
| DOI: 10.21608/mjb.2020.462408 | ||
| Authors | ||
| Basma El-Sayed* 1; Abdel-Fattah, G.M1; Ali, M.S2 | ||
| 1Botany Department, Faculty of Science, Mansoura University, 35516 Mansoura City, Egypt | ||
| 2Microbiology Department, Faculty of Science, Ain Shams University, Egypt | ||
| Abstract | ||
| Bioremediation of toxic phenol is a cost-effective, efficient and eco-friendly method. The target of this research was to isolate potent degrading phenol bacteria, optimize the biodegradation and detect the pathway. A phenol degrading bacterium (B1) was isolated from acontaminated soil sample from amechanical shop, Cairo, Egypt. The isolate showed complete degradation of phenol as a sole carbon and energy source. The isolate was identified using 16S rRNA gene sequence and submitted to Genbank and identified as Pseudomonas putida strain B1 under accession number MT675100. The isolated strain exhibited the maximal and complete degradation of the phenol within 100 mg/l phenol concentration, initial pH of 7 and 30°C incubation temperature after 72 h incubation. P. putida strain B1 degraded phenol up to 800 mg/l. UV/VIS spectrophotometric analysis indicated that P. putida strain B1 degraded phenol via meta-ring cleavage pathway that revealed using the formation of 2-hydro-xymuconate. | ||
| Keywords | ||
| Biodegradation; Phenol; Meta pathway; Pseudomonas | ||
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