SENSITIVITY OF SOME CELL LINES TO ADAPT PESTE DES PETITS RUMINANTS’ VIRUS FOR VACCINE PRODUCTION | ||
| Assiut Veterinary Medical Journal | ||
| Article 45, Volume 71, Issue 187, October 2025, Pages 575-584 PDF (854.72 K) | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.2025.359241.1579 | ||
| Authors | ||
| MOHAMED H. KHODEIR; AFAF A. ABD EL WAHAB; HANAN S. ABD EL-RAOOF; HANAN M.S. EL-ZAWAHRI; ABEER A. TAMAM; MOHAMED M. YOUSSEF; DALIA A.M. ABD EL-MOATY* | ||
| Department of Rinderpest Vaccine Research (DRPVR), Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt | ||
| Abstract | ||
| Preparation of peste des petits ruminants virus (PPRV) vaccine is traditionally carried out by adapted virus in Vero cells with typical cytopathic effects (CPEs). Alternative cell lines would be evaluated for the virus propagation as Baby Hamster Kidney cells (BHK) and Madin-Darby Bovine Kidney cells (MDBK). We aim to establish an alternative cell line for production of live attenuated PPR vaccine in case of loss or unavailable required Vero cells for such purpose. The Nigerian PPR virus (N75/1) was passaged 10 successive times in Vero, BHK21 and MDBK cell lines followed by virus titration and growth curve studies. Cell rounding, cytoplasmic vacuolation and syncytia formation were the most noticeable characteristic CPE for PPRV. The PPRV multicycle growth analysis in the supernatant of cells revealed a constant increase in the virus quantity that indicates PPRV adaptation to the used cell lines having virus titer 7, 6 and 6.5 log10 Tissue culture infective dose 50 (TCID50 ) within 120; 144 and 96 hours post cell infection for Vero, BHK21 and MDBK respectively. The obtained findings suggest that Vero, BHK21 and MDBK cells can be reliable ulternatives for propagation of PPRV for vaccine production. | ||
| Keywords | ||
| Peste des petits ruminants; cell lines; virus titration; growth kinetics | ||
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