Genomic characterization of antimicrobial resistance and mobile elements in Klebsiella quasipneumoniae IIEMP3 isolated from Indonesian tempeh | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 28 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.375690.2698 | ||
| Authors | ||
| Noor Andryan Ilsan* 1; Elfira Maya Sari1; Maulin Inggraini1; Ria Amelia1; Melda Yunita2 | ||
| 1Department of Medical Laboratory Technology, STIKes Mitra Keluarga, Indonesia | ||
| 2Department of Microbiology, Faculty of Medicine, Universitas Pattimura, Ambon, Maluku, Indonesia | ||
| Abstract | ||
| Background: Tempeh is an Indonesian popular and traditional fermentation food from soybean. Tempeh has been known inhabited by various bacteria, despite fermented mostly by Rhizophus sp. fungus. Previous study using whole genome analysis of Klebsiella sp. from Indonesian tempeh, Bogor, West Java, proved that this isolate was different to those medical isolates, also harmless to human due to the absence virulence gene. The aim of this study was to understand the genomic accessories related to antimicrobial resistance in Klebsiella quasipneumoniae IIEMP3 isolated from Indonesian tempeh. Methods: We analyzed whole genome sequence of this isolate using bioinformatic tools, including resistant genes, ori plasmid detection, mobile genetic element detection, and plasmid comparison. Results: In this study, whole genome analysis of K. quasipneumoniae IIEMP3 from Indonesian tempeh showed that this isolate contained intrinsic antimicrobial resistance genes, including fosA for fosfomycin resistant and, oqxA, oqxB for quinolone resistant in contig 37 and 36, respectively. These resistance genes belong to chromosomal region, since it was not found plasmid origin in these contigs. Plasmid origin has also been detected i.e. IncFIB(K) and InfII(K) in contig 45 and 2, respectively. Moreover, mobile genetic elements were found in this isolate, including ISKpn24, ISEcI10, ISEhe3, IS102, and ISKpn14 in contig 45 IncFIB(K) plasmid. Interestingly, all of the resistant gene seems to be located in chromosomal and not correlated to the dissemination of the resistant genes. Conclusions: These resistance genes are not situated on the same plasmid InFIB(K), which contains a large number of mobile genetic elements for genetic transfer. | ||
| Keywords | ||
| Tempeh; Genomic accessories; antimicrobial resistance; Klebsiella quasipneumoniae; Plasmid | ||
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