The Possible Protective Role of Pumpkin Seed Oil in Improving Tongue Mucosal Damage Induced by Artificial Sweeteners Aspartame (Histological study)
Elemam, H., Mohamed, A. (2025). The Possible Protective Role of Pumpkin Seed Oil in Improving Tongue Mucosal Damage Induced by Artificial Sweeteners Aspartame (Histological study). EKB Journal Management System, 4(4), 3297-3313. doi: 10.21608/erurj.2025.371772.1246
Heba Fathi Elemam; Abeer Abd El Maksoud Mohamed. "The Possible Protective Role of Pumpkin Seed Oil in Improving Tongue Mucosal Damage Induced by Artificial Sweeteners Aspartame (Histological study)". EKB Journal Management System, 4, 4, 2025, 3297-3313. doi: 10.21608/erurj.2025.371772.1246
Elemam, H., Mohamed, A. (2025). 'The Possible Protective Role of Pumpkin Seed Oil in Improving Tongue Mucosal Damage Induced by Artificial Sweeteners Aspartame (Histological study)', EKB Journal Management System, 4(4), pp. 3297-3313. doi: 10.21608/erurj.2025.371772.1246
Elemam, H., Mohamed, A. The Possible Protective Role of Pumpkin Seed Oil in Improving Tongue Mucosal Damage Induced by Artificial Sweeteners Aspartame (Histological study). EKB Journal Management System, 2025; 4(4): 3297-3313. doi: 10.21608/erurj.2025.371772.1246

The Possible Protective Role of Pumpkin Seed Oil in Improving Tongue Mucosal Damage Induced by Artificial Sweeteners Aspartame (Histological study)

ERU Research Journal
Volume 4, Issue 4, October 2025, Pages 3297-3313    PDF (981.74 K)
Document Type: Original Article
DOI: 10.21608/erurj.2025.371772.1246
Authors
Heba Fathi Elemam* 1; Abeer Abd El Maksoud Mohamed2
1Oral Biology Department, Faculty of Dentistry, Egyptian Russian University
2Lecturer of Oral Biology, Department of Oral Biology, Faculty of Dentistry, Fayoum University, Fayoum, Egypt
Abstract
Aspartame is a low-calorie artificial sweetener commonly used in a variety of processed foods; accompanied by degenerative changes in different tissues. The present study designed to evaluate the possible protective role of pumpkin seed oil in improving tongue mucosal damage induced by artificial sweetener aspartame in albino rats histologically. Thirty adult male albino rats (180 -200 gm) divided randomly into three groups (n=10). Group I (Control): rats received 1 ml distilled water. Group II: rats received aspartame (250 mg/kg in distilled water) orally. Group III: rats received dose of aspartame as the group II, additionally topical application of pumpkin seed oil. After four weeks, rats were euthanized and tongues were dissected and cut longitudinally, for histological and ultrastructure microscopic examination. Histological examination revealed abnormalities in the morphology of tongue papillae at group II represented by hyperplasia (acanthosis), cellular pleomorphism, nuclear hyperchromatism, congested blood vessels, and brown positive immunoreaction of only some basal epithelial cells to Ki67. This confirmed by ultrastructure microscopic examination which revealed a disfigurement of tongue papillae. In contrast, minimal changes appeared at group III. Conclusion, aspartame induced marked morphological changes at the tongue's mucosa of albino rats. pumpkin seed oil has a protective role against these effects.
Keywords
Aspartame; pumpkin seed oil; tongue papillae
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