Protein and DNA fingerprints of Some Multidrug-Resistant Bacteria | ||
| Mansoura Journal of Biology | ||
| Volume 45, Issue 4, December 2019, Pages 14-21 PDF (655.55 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462672 | ||
| Authors | ||
| Musaab Abd Muishin1; Mohammed Nather Maroof2; Y. A. Osman* 1 | ||
| 1Botany Department, Faculty of Science, Mansoura University, Egypt | ||
| 2Dpartment of Biology, College of Education, Tikrit University, Iraq | ||
| Abstract | ||
| Multidrug resistance is a major threat to the fighting of infectious bacterial pathogens. This is because they turn the current generations of antibiotics into obsolete non-effective compounds. Therefore the search for new antibacterial compounds is complicated because of the price tag and the time needed for marketing. Nine pathogenic bacteria were tested for their resistance to 19 different antibiotics and studied at molecular levels using protein banding patterns, DNA fingerprinting, when grown at optimum and elevated temperatures. The eight strains belonging to five genera of bacteria were resistance to the 19 drugs was evident with Aeromonas sp., E. coli, Klebsiella pneumonia, Proteus mirabilis, and Pseudomonas aeurginosa. Only Klebsiella pneumonia strain 1 showed sensitivity to the meropenem disc saturated with 10ug of the drug. The protein banding patterns discovered the minute differences between the two isolates of Psuedomonas, Klebsiella and E. coli. Moreover, the DNA fingerprints using RAPD-PCR distinguished between the two Klebsiellia strains and E. coli strains. In conclusions the use of molecular biology tools can be helpful in distinguishing between closely related strains of the same bacterial pathogen species | ||
| Keywords | ||
| Multidrug; resistance; bacteria; antibiotic; fingerprinting | ||
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