Comparison and Differentiation of Oral Microbial Flora from Healthy and Diabetic Individuals | ||
| Mansoura Journal of Biology | ||
| Volume 45, Issue 4, December 2019, Pages 22-29 PDF (692.96 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462673 | ||
| Authors | ||
| Ibrahim K. Ahmed1; Mohamed A. Abaas2; Yehia A. Osman* 2 | ||
| 1Graduate from the University of Baghdad, Iraq | ||
| 2Department of Botany, Faculty of Science, Mansoura University 35516, Egypt. | ||
| Abstract | ||
| Oral microbial flora constitutes an important part of the total human microbiome and any imbalance of this community is directly reflected upon the oral and whole-body health and disease status. Diabetes mellitus affects the mouth environment in terms of the flow of saliva and hence nutrients availability for mouth microbes. Studying and comparing the mouth cavity microbial flora of healthy and diabetes represented a mystery needed to be discovered. The use of the available molecular techniques such as protein and DNA fingerprinting were adopted to answer and expanded our knowledge about this part of the human body environment. The molecular methods were not only superior to phenotypic methods of bacterial identification but they were faster and conclusive. It is highly important to note the ability of the molecular techniques to distinguish between strains of the same species; either at the protein or DNA levels. Moreover, it is an obligation to state these methods works well with cultivable bacteria and other methods depending on DNA sequencing and 16S rRNA would be important for non-cultivable microbes . | ||
| Keywords | ||
| Oral; microbiomes; diseases; molecular and phenotypic | ||
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