Bacterial Status of Some Drinking Waters from Dakahlyia and Alexandria Governorates of Egypt | ||
| Mansoura Journal of Biology | ||
| Volume 45, Issue 4, December 2019, Pages 37-45 PDF (671.65 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462680 | ||
| Authors | ||
| Hayder G. Wajer1; H. ElSheshtawy2; Yehia A. Osman* 1 | ||
| 1Botany Department, Faculty of Science, Mansoura University, 35516, Egypt | ||
| 2Agriculture Genetic Engineering Research Institute, ARC, Giza, Egypt | ||
| Abstract | ||
| Availability of sanitized quality water is one of the basic rights of humans worldwide. The international standards require the drinking waters to be odorless, tasteless, and colorless besides being free of pathogenic microbes and toxic heavy metals. Using traditional microbiological techniques twelve bacterial colonies were isolated and identified from eight drinking water samples. Molecular biology tools such as random amplified polymorphic deoxyribonucleic acid fingerprinting, protein banding patterns, sequencing the gene encodes for a conserved region of the 16S rRNA, and Matrix-Assisted Lasar Desorption Ionization were used for identification of the multidrug-resistant bacterial isolates from drinking waters. All bacterial isolates belong to three genera named E. coli, Staphylococcus epidermis, and Bacillus halo... The accuracy of molecular techniques to the speciation of the bacterial isolates was beyond doubts. Moreover, Matrix-Assisted Lasar Desorption Ionization proved to be faster, highly accurate and cheaper to perform than other molecular biology techniques; considering the availability of the instrument and the existence of the bacterial spectra in its library. | ||
| Keywords | ||
| Drinking water; Bacteria; MALDI; Molecular biology | ||
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