Phenotypic and Genotypic Characterization of Biofilm Formation among Staphylococcus aureus Isolates | ||
| Mansoura Journal of Biology | ||
| Volume 44, Issue 3, September 2019, Pages 1-7 PDF (789.38 K) | ||
| Document Type: Research Article | ||
| DOI: 10.21608/mjb.2019.462684 | ||
| Authors | ||
| Alkubaisi, A.R* 1; Abdel-Fattah, G.M1; El Degla, H.E2; Zeid, M.S3 | ||
| 1Department of Botany, Faculty of Science, Mansoura University, Egypt | ||
| 2Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt. | ||
| 3Lecturer of pediatrics Infectious disease and Malnutrition Unit | ||
| Abstract | ||
| (The main objective of this study was the characterization of S. aureus clinical isolates collected from different patients admitted to Mansoura hospitals. Then, this study was extended to detect the ability of these isolates to form biofilm by genotypic and phenotypic methods. Fifty-two clinical S. aureus isolates were collected from patients admitted in different intensive care units (ICU) of Mansoura University Hospitals. Antibiotic susceptibility pattern of S. aureus was tested by disc diffusion method. The antibiotic susceptibility of different isolates of S. aureus to different tested antibiotics showed that the tested isolates were highly resistant to vancomycin (92.30%) and ciprofloxacin (61.5%) and the lowest sensitivity was detected with amoxicillin, penicillin, cefotaxime and meropenem. Results of the PCR technique on 18 S. aureus strains on 9 strains were biofilm producer and other 9 were non produced biofilm based on both CRA and MTP methods showed that 9 strains were positive for icaA and icaD. The PCR product bands for all positive strains were 188-bp band for the icaA gene and 198-bp band for the icaD while bap was not detected in any strain. | ||
| Keywords | ||
| Antibiotic sensitivity; Staphyllococcus aureus; Biofilm formation; IcaA and IcaD genes | ||
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