Impact of some cationic bichalcophene compounds on bacillus species | ||
| Mansoura Journal of Biology | ||
| Volume 44, Issue 3, September 2019, Pages 25-33 PDF (904.88 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462690 | ||
| Authors | ||
| Sally M. Elmogy* 1; Mohamed A. Ismail2; Rabeay Y. Hassan3; Ashraf A. Elsayed1 | ||
| 1Botany Department, Faculty of Science, Mansoura University, Elgomhouria St.,35516 Mansoura City, Egypt | ||
| 2Chemistry Department, Faculty of Science, Mansoura University, Elgomhouria St.,35516 Mansoura City, Egypt | ||
| 3Center for Materials Science, Zewail City of Science and Technology, 6th October City, 12588 Giza, Egypt | ||
| Abstract | ||
| Bacterial resistance to multiple antibiotics is a great health problem; hence different antibacterial agents need to be constantly discovered. The antimicrobial activities of the newly synthesized fluorophenyl-2,2′-bichalcophene derivatives were evaluated against Bacillus sp. The impact of these bichalcophenes on Bacillus sp. was studied at the molecular level via SDS-PAGE and RAPD-PCR methods. The investigated bichalcophenes are shown to have a potent suppressing effect on Bacillus sp. growth. Among the investigated bichalcophenes, fluorinated thiophene-furan derivatives MA-1115 and MA-1116 showed the highest activity against Bacillus sp. bacterial strain.Also it can be observed that, the bichalcophenes MA-11156, MA-1114, and MA-1113 exhibited a good antimicrobial activity against Bacillus sp., and were more higher in activity than that of the parent compounds MA-0944 (bithiophene derivative) and MA-0947 (bifuran derivative). The minimum inhibitory concentration (MIC) values for the two selected fluoroarylbichalcophenes MA-1115 and MA-1116 were 32 and 64 µM, respectively. Over a period of 7 days Bacillus sp. did not develop resistance to the two selected bichalcophenes at higher concentrations (2, 3x MIC in case of MA-1115 and 3x MIC in case of MA-1116). The biomarker assay detecting the protein changes based on SDS-PAGE profile showed that, there were six bands disappeared from some concentrations after treatment with 8 and 16 µM of MA-1115 and 16 and 32µM of MA-1116. On the other hand, three new bands appeared comparing to untreated bacteria. Also the genetic changes based on RAPD-PCR manipulation of compounds MA-1115 and MA-1116 manifested a polymorphic pattern when treated and untreated bacteria were compared. The impact of compounds MA-1115 and MA-1116 was obviously strong on genetic structure and protein of Bacillus sp. based on RAPD-PCR manipulation and SDS-PAGE profile, so these two bichalcophene compounds could be recommended for their application as effective antibacterial agents. | ||
| Keywords | ||
| bacterial resistance; bichalcophenes; antimicrobial activity; Bacillus sp | ||
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