Acclimatization of Bacteria to Bladder Cancer Tissues | ||
| Mansoura Journal of Biology | ||
| Volume 44, Issue 3, September 2019, Pages 55-64 PDF (814.81 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462695 | ||
| Authors | ||
| Omar A. Al-Halboosi1; Ahmed A. Shokier2; Yehia A. Osman* 1 | ||
| 1Botany Department, Faculty of Science, Mansoura University, 35516, Egypt | ||
| 2Urology and Nephrology Center, Mansoura University, Egypt | ||
| Abstract | ||
| Cancer (including bladder cancer) is not a simple disease due to the multiple reasons responsible for its onset in humans; which vary from socioeconomic, environmental, chemical, to genetic reasons. Bladder cancer occupies eighth place amongst the newly diagnosed malignant cases worldwide. Moreover, men diagnosed with bladder cancer are four times higher than women. Early and non-invasive techniques for the diagnosis of bladder cancer are encouraged to avoid medical complications lengthy and expensive tests. Building a bacterial fingerprint of bladder cancer should contribute to the easiness of the diagnosis process and hence speed up the treatment. Standard bacteriological techniques were used to isolate bacteria from 10 samples of cancer tissues collected from the Urology and Nephrology Centre at Mansoura University, Egypt. The bacterial isolation was followed by classical and molecular identification of twenty bacteria. Bacterial protein fingerprinting screening reduced the 20 isolates to five bacteria which were further identified using 16S rRNA gene sequencing, RAPD-PCR DNA fingerprinting, and MALDI mass spectrophotometry analysis. Then all isolated grouped into three bacterial genera namely E. coli (three isolates), P. aeruginosa, and Klebsiella pneumonia. MALDI mass spectrophotometer proved to be the easiest, faster, convenient and cheapest way of bacterial identification. | ||
| Keywords | ||
| Bacteria; bladder; cancer; fingerprinting; and MALDI | ||
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