Biological control of chocolate spot disease in Vicia faba L. using the synergism among certain bio-agents | ||
| Mansoura Journal of Biology | ||
| Volume 43, Issue 2, June 2019, Pages 1-13 PDF (716.82 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462698 | ||
| Authors | ||
| Hoda M. Soliman* 1; K. M. Abd El-Hai1; Eman O. Zedan2 | ||
| 1Botany Department, Faculty of Science, Mansoura University, Egypt | ||
| 2Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt | ||
| Abstract | ||
| All of twenty five Botrytis fabae isolates isolated from naturally infected faba bean plants collected from five different districts in Dakahlia governorate were found to be pathogenic and caused typical symptoms of chocolate spot disease while, the isolate B4 was the most aggressive. Twenty five isolates of Trichoderma related to four species (T. viride, T. harzianum, T. hamatum, T. album) were screened for the antagonistic activity against the most aggressive B. fabae B4. All the twenty five isolates of Trichoderma showed some sort of antagonistic activity against the tested B. fabae B4 while The isolate T. viride T2 was the most potent antagonist to the pathogen B. fabae B4 followed by T. harzianum E3. Among the three bio-agents; T. viride T2, T. harzianum E3 and yeast extract, yeast extract was the most potent antagonist against B. fabaegiving the highest %inhibition of growth parameters and sporulation followed by T. harzianum E3 then T. viride T2. Both non-volatile and volatile metabolites of T. viride T2 and T. harzianum E3 significantly inhibited the linear growth of B. fabae. The non–volatile metabolites of both Trichoderma species were more potent in inhibition of mycelial growth of B. fabae than the volatile metabolites. Ethyl acetate extracts of both T. viride T2 and T. harzianum E3 had antifungal activity and inhibited the growth and sporulation of B. fabae while, T. harzianum E3 was the most effective. Both of T. viride T2 and T. harzianum E3 produced cellulolytic and chitinolytic activity more than B. fabae. On the other hand, B. fabae produced much more proteolytic activity than them. There was no significant difference in amylolytic activity of all the three tested fungi. The green house experiment indicated that the treatment of Faba bean seeds and foliar spraying with the combination of Rhizobium, T. viride T2 and yeast was the best bio-agents combination in reduction of chocolate spot disease severity while, the individual treatment with T. viride T2 or T. harzianum was the most effective against disease incidence. The combination of Rhizobium, T. viride T2 and yeast and the combination of Rhizobium, T. harzianum E3 and yeast gave the highest increase in productivity traits. | ||
| Keywords | ||
| Rhizobium; Trichoderma; yeast; B. fabae; faba bean | ||
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