Endophytes isolated from wheat and phragmites and their effect on maize grain priming | ||
| Mansoura Journal of Biology | ||
| Volume 43, Issue 2, June 2019, Pages 14-23 PDF (862.93 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462700 | ||
| Authors | ||
| Ashraf Elsayed; Marwa Magdy Fawzy* ; Ahmed Shawkey Gebreil; Amr Mohammed Mowafy | ||
| Botany Department, Faculty of Science, Mansoura University, 35516 Mansoura City, Egypt | ||
| Abstract | ||
| The use of plant growth promoting bacteria (PGPB) is considered as a main part of today's sustainable agriculture practices. The intended target of this study was to use PGPB to stimulate maize priming. The endophytes inhabiting both Wheat and Phragmites were the source of the desired isolates. These plants were collected and then surface sterilized and the endophytes were isolated from their different parts. The obtained isolates were tested as priming agents to stimulate maize growth. Out of the twenty-one obtained isolates, the three isolates E1S2, MK2R2 and B2L2 stimulated the growth of maize grains to 98%, 95% and 95% compared to 85% for water and 80% for media priming. These isolates have been molecularly identified as Enterobacter cloacae, Klebsiella michiganensis and Bacillus subtilis respectively. Additionally, other germination vigor parameters such as length of plant root and hypocotyl in addition to number of lateral roots increased for various degrees in response to these biogenic treatments. The obtained isolates Enterobacter E1S2, Klebsiella MK2R2 and Bacillus B2L2 were found to be the best maize growth promoting bacteria according to the results obtained from the preliminary laboratory experiments shown in this study. These results pave the way for studying the plant growth promoting criteria of these isolates in order to use them to improve maize growth and metabolism, a study that is being carried out in the field conditions. | ||
| Keywords | ||
| Bacillus; Endophytes; Enterobacter; maize; germination percentage; Klebsiella | ||
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