Cytokine profiling in mice suffering fatty liver pre administered with probiotics | ||
| Mansoura Journal of Biology | ||
| Volume 43, Issue 2, June 2019, Pages 67-74 PDF (796.55 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462718 | ||
| Authors | ||
| Sherif H. Abdeen* 1; Amany A. Latief1; Medhat A. El-Daker2 | ||
| 1Zoology Department, Faculty of Science, Mansoura University | ||
| 2Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University | ||
| Abstract | ||
| The pro-inflammatory cytokines, as Tumor necrosis factor alpha (TNF-α) that being an endogenous pyrogen, that can cause apoptotic cell death, inflammation, and intervene the arrival of assortment cytokines such as Interleukin six (IL-6), Interleukin one beta (IL-1β) and Interleukin eight (IL-8) by activate macrophages. Dys-regulation, specifically excessive production of TNF-α has been detect in an assortment of many disorders like atherosclerosis, malignancy, and inflammatory bowel diseases. Probiotics are live bacteria, for example, Lactobacilli and Bifidobacteria; when consumed by people as adequate doses, these bacteria present a medical advantage on the host. This study estimated the efficacy and presumable action of two strains (L. acidophilus and B. animalis) to reduce fat accumulation in the liver of C57BL/6 male mice with diet-induced fats. Animals were divided into 4 groups: Group I; ten animals will receive normal diet for two weeks. Group II; ten animals will receive normal diet for the same period with probiotics. Group III; ten animals were subjected to high fatty diet; the high-fat diet contained 75% fat (butter) for the same period. Group IV: ten animals were subjected to high fatty diet for the same period with probiotics. TNF-a, IL-6 and IL-10 had been analyzed by enzyme-linked immunosorbent assay (ELISA), also lipogram and liver function tests were done. In this study the serum levels of IL-10 did not increase significantly in mice fed on high fatty diet in comparison with those on normal diet, while its level increase in animals which have probiotics introduced with fatty diet. In the present study the mean levels of IL-6 and TNFα, also liver enzymes and serum lipids were elevated in mice fed on high fatty diet in comparison with those on normal diet, while its level decreased in animals which had probiotics introduced with fatty diet. | ||
| Keywords | ||
| Cytokines; probiotics; Fatty Liver; Steatosis; Interleukins | ||
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