Mycolytic activity of Trichodermia viride against Macrophomina phaseolina associated with soybean seeds | ||
| Mansoura Journal of Biology | ||
| Volume 42, Issue 1, March 2019, Pages 1-11 PDF (662 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.462738 | ||
| Authors | ||
| Khalid M. Ghoneem* 1; Noha M. El-Dadamony1; Gamal M. Abdel-Fattah2 | ||
| 1Seed Pathology Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt. | ||
| 2Botany Department, Faculty of Science, Mansoura of University, | ||
| Abstract | ||
| The soybean seeds were subjected to health evaluation using standard blotter and agar plate techniques, for detection of seed-borne mycoflora. Thirty-one fungal species comprising nineteen genera were detected. Several pathogenic and storage fungi were observed by both methods. Among the fungal pathogens recovered, Macrophomina phaseolina was found predominant (up to 27.3%) and it was used as test pathogen for further studies. Antagonistic properties of seven seed-borne Trichoderma isolates were evaluated against M. phaseolina through dual culture technique. Trichoderma T44sn isolate markedly retarded the pathogen growth, reaching 81.23% after 5 days of incubation. Eight days later the pathogen was completely overgrown by T44sn mycelia and sclerotia formation was lacking compared to the control plates. The antagonistic effect of the cell-free extracts of Trichoderma isolates against M. phaseolina by agar well diffusion assay proved that T44sn isolate was the potent antagonistic isolate against M. phaseolina, which generated 2.47 cm inhibition zone of the pathogen growth, followed by T42sn 2.20 cm. Trichoderma sp. T44sn strain was Molecularly identified using 18S rRNA as T. viride. Its chitinase activity was found to be 0.20 U, this low activity on non-specific medium for the enzyme production reflecting the mycolytic potentiality of the present T. viride. Hence, we recommend further investigations on the use of T. viride as a biological agent for use as biocontrol agents against plant pathogens.. | ||
| Keywords | ||
| : Seed-fungi; soybean; Macrophomina phaseolina; Trichoderma viride; biological control | ||
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