Prevalence and Molecular Identification of Blastocystis Subtypes in Sheep in Salah al-Din Province, Iraq | ||
| Egyptian Journal of Medical Microbiology | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 July 2026 PDF (459.09 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.433677.1943 | ||
| Author | ||
| Zuhair M. Abed* | ||
| Salah Al-Din Education Directorate, Ministry of Education, Tikrit, Iraq | ||
| Abstract | ||
| Background: Blastocystis sp. is among the most common and important pathogens identified in humans and various type of animals hosts including sheep, cattle, and goat, with a global distribution. Objective: determine the prevalence and molecular subtypes of Blastocysits in sheep from rural areas of Tikrit, Iraq. Methodology: 29 stool specimens (approximately 7-10 g per specimen) were taken from sheep suffering from diarrhea, as well as 154 specimens from sheep that did not show any clinical signs, samples were collected from different rural areas of Tikrit city, Iraq for the period from January to June 2025, DNA extraction from samples was performed by taking about 200 mg of fecal sample, by stool DNA kit. Results: The result of the PCR (Polymerase Chain Reaction) examination showed that the overall infection rate reached 16 (8.74%) out of 183 fecal samples were positive to Blastocystis, two subtype of Blastocystis, ST10 and ST5, were detected in this study, The sequencing results were recorded in the Gen Bank with the following numbers: PV876305.1, PV876306.1, PV876307.1, PV876308.1 PV876309.1. Conclusion: the identification of two Blastocystis subtypes in this study indicates the presence of genetic diversity among the detected isolates and this result confirms the importance of continuous molecular surveillance to understand the epidemiology and transmission routes. | ||
| Keywords | ||
| Blastocystis sp; sheep; PCR; Sequencing; ST5; ST10 | ||
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