Protocol for Guava propagation by Tissue Culture Techniques | ||
| Damietta Journal of Agricultural Sciences | ||
| Volume 4, Issue 3, August 2025, Pages 56-62 | ||
| Document Type: Original research papers | ||
| DOI: 10.21608/djas.2025.462829 | ||
| Authors | ||
| Ahmed Abdelsamee Attia* 1; El Refaey Eldengawy2; doaa mahmoud delgiar3 | ||
| 1kafr Elsheikh, desouq, kafr El arab | ||
| 2Horticulture Department, Faculty of Agriculture, Damietta University | ||
| 3قسم البساتين كلية الزراعة جامعة كفر الشيخ | ||
| Abstract | ||
| This study aimed to investigate the best protocol for Proliferation and rooting of guava nodal explants obtained by tissue culture techniques. The sterilization process was done through immersing the nodes into70% (v/v)ethanol for1minute then in 0.05%mercuric chloride(HgCl2)for6minutes then clorox (NaOCl) at40% for15minutes. The control of phenolic compounds exudation was done by collection of explants in spring and they were cultured vertically. Then the explants were immersed in a mixture of ascorbic acid150mg/l, citric acid100mg/l and0.5%polyvinylpyrrolidone(PVP). In addition ascorbic acid150mg/land citric acid100mg/l mixture were added to the culture media. The elongation treatment which gave highest average number of shoot is the MS media supplied with 1 or 1.5 mg/lBA. Moreover, adding (1 mg/lBA + 0.2 mg/lIBA) and (1.5 mg /lBA+ 0.5 mg/lIBA) to MS media gave the best effect on average length of explant and average number of leaves. While the best results for rooting experiment were obtained from MS medium at half strength supplemented with1mg/lIBA. The average root number (2.8) and average root length(3cm). The final step was acclimatization which involved moving guava plantlets from aseptic culture conditions to greenhouse condition. The results revealed that the plantlets were transferred to greenhouse conditions after acclimatization for2month and the survival percentage was65%. | ||
| Keywords | ||
| guava; tissue culture؛ IBA؛ NAA؛ rooting | ||
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