Prevalence of bla TEM and bla KPC Resistance genes in Klebsiella pneumoniae Isolated from animal sources | ||
| Assiut Veterinary Medical Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 31 October 2025 | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.2025.404972.1802 | ||
| Authors | ||
| Abdullah Rajab* ; Abdul Kareem Alyassari | ||
| Department of Microbiology, College of Veterinary Medicine, AL-Qasim Green University, Babylon 51013, Iraq. | ||
| Abstract | ||
| Klebsiella pneumoniae is a Gram-negative, encapsulated, and opportunistic pathogen that poses a significant threat to animal health due to its ability to acquire antibiotic resistance genes. This study aimed to molecularly detect the presence of bla-TEM and bla-KPC beta-lactamase genes, along with the 16S rRNA gene, in K. pneumoniae isolates collected from animal sources. A total of 20 isolates were obtained from animals showing clinical signs of infection, specifically diarrheic chickens and neonatal calves. All isolates were identified using conventional microbiological techniques and confirmed by Polymerase Chain Reaction (PCR) analysis. The results revealed that 100% of the isolates carried both the 16S rRNA, while 40% were positive for the bla-TEM genes, and 45% were positive for the bla-KPC gene. These findings indicate a high prevalence of extended-spectrum beta-lactamase (ESBL) genes in K. pneumoniae strains isolated from animals, suggesting the potential role of animal reservoirs in the emergence and dissemination of antimicrobial resistance. The use of molecular diagnostic methods such as PCR, alongside automated identification systems like VITEK 2, provides an effective approach for the rapid and accurate detection of antibiotic resistance genes in veterinary microbiology. Continued surveillance is necessary to monitor resistance trends and inform control strategies within a One Health framework. | ||
| Keywords | ||
| K. pneumoniae; antibiotic resistance; animal; bla-TEM and bla-KPC | ||
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