In vitro Cytotoxicity of Plantaricin MS9 Produced by Lactiplantibacillus plantarum MS, Isolated from the Mediterranean Sea, Egypt | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Volume 29, Issue 5, September and October 2025, Pages 3509-3530 PDF (678.12 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2025.429370.6710 | ||
| Authors | ||
| Moustafa Elnaggar1; Mennatullah Lamada2; Amira Hamdan* 3 | ||
| 1Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt | ||
| 2Botany and Microbiology Department, Faculty of Science, Alexandria University | ||
| 3Oceanography Department, faculty of Science, Alexandria University | ||
| Abstract | ||
| The present study evaluates the cytotoxic activity of the bacteriocin produced from Lactiplantibacillus plantarum MS, a novel marine lactic acid bacterium. The results showed that the crude bacteriocin displayed a wide antibacterial spectrum against some selected bacterial strains. The purified bacteriocin has a molecular weight of 3.5 kDa detected by SDS-PAGE and a molecular mass of 2995.6 Da quantified by LC/MS. Based on amino acid sequencing, it was concluded that plantaricin MS9 is a new class IId bacteriocin with a bacteriostatic mode of action. Biochemical characterization of plantaricin MS9 confirmed that it is thermostable over a broad range of temperatures (50- 100°C), retained its activity at acidic and alkaline pH values, and was strongly inhibited by proteolytic enzymes. Furthermore, plantaricin MS9 exhibited strong antiproliferative activity and detrimental effects against three cancer cell lines: colon cancer (Caco-2), liver cancer (HepG2), and breast cancer (Mcf-7), with inhibition rates of 93, 95, and 91%, respectively. In conclusion, the collected data revealed that plantaricin MS9 is of potential interest for future applications in the pharmaceutical industry. | ||
| Keywords | ||
| Cytotoxic activity; Antimicrobial activity; Bacteriocin; Plantaricin MS9; Lactiplantibacillus plantarum MS | ||
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