UTILIZATION OF SOME AGRO-INDUSTRY WASTES FOR PRODUCTION OF FUNGAL LIPASE | ||
| Mansoura Journal of Biology | ||
| Volume 42, Issue 1, March 2019, Pages 12-17 PDF (521.3 K) | ||
| Document Type: Research Article | ||
| DOI: 10.21608/mjb.2019.463317 | ||
| Authors | ||
| Mohamed Magdy EL-Metwally* 1; Abdel-Fattah, G. M2; Youssef M.M. Mohammed3; Al-Amri, M. S3; El-Khatieb, D.K3 | ||
| 1Department of Botany and Microbiology, Faculty of Science, Damnhour University | ||
| 2Department of Botany, Faculty of Science, Mansoura University, Egypt | ||
| 3Department of biology, College of Science and ART, Shaqra University, Saudi Arabia | ||
| Abstract | ||
| Fungi are used in several industrial processes, such as production of enzymes. Importance of enzymes is increasing especially microbial lipases which are of indefinite industrial purposes. Solid state fermentation is an economical alternative for large scale production of enzymes which produced by fungi. By using solid state fermentation, the production of extra- cellular lipase from filamentous fungi isolated from soil and the oil contaminated soil using agricultural wastes has been studied. Seven fungal strains were isolated from twenty soil samples and then tested for lipase production. Among these fungi, Aspergillus flavipes was identified and selected as highest lipase producer using Nigella sativa as a waste among seven agricultural wastes. The optimum conditions for lipase production were pH 7.2, temperature 28°c and 6 day incubation period. Maltose and yeast extract were the best carbon and nitrogen source respectively. | ||
| Keywords | ||
| Solid state fermentation; Agro-industrial wastes; Enzyme production; Optimization of lipase production; Aspergillus flavipes | ||
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