ISOLATION AND IDENTIFICATION OF ANTIMICROBIAL PRODUCING BACTERIA FROM MARINE SEDIMENT SAMPLE | ||
| Mansoura Journal of Biology | ||
| Volume 42, Issue 1, March 2019, Pages 35-42 PDF (716.51 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.463330 | ||
| Authors | ||
| Attiya H. Mohamedin; Ahmed Abd Elrazak; Amany S. Hegazy* | ||
| Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt | ||
| Abstract | ||
| Searching for novel antimicrobial agents with novel target or mechanism of action become a global demand as the bacterial resistance against antibiotics is in constant increase and this has a great effect on the mortality and increase the cost of treatments associated with control of bacterial infection. Marine natural products gained greet attentions as they have shown many interesting activities such as: anti-microbial, anticancer, anti-coagulant, anti-inflammatory, anti-diabetic, cytotoxic effect and other pharmacological activities. Marine microorganisms are able to produce unusual bioactive metabolites as they live in biologically competitive environment and they have to adapt to extreme conditions such as: pH, temperature, high pressure and salinity. In this work, marine sediment sample was collected from Red Sea, Egypt. From this sample, bacteria were isolated by pour plate method using Zobell 1/4 strength media at two different temperatures, 37°C and 28°C. All the 25 different bacterial isolates were screened for antimicrobial material production using disc diffusion method. The most potent antimicrobial agent was identified by the means of biochemical tests and molecular identification using 16S rRNA sequence analysis. The isolate was found to be belonging to Pseudomonas genera. | ||
| Keywords | ||
| Pseudomonas; Bacteria; Antibiotic; Biochemical tests; Molecular Identification; Marine sediment | ||
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