Production of Polyunsaturated Fatty Acids (PUFAs) From Some Marine Microalgae with Special Emphasis on Eicosapentaenoic Acid (EPA)” | ||
| Mansoura Journal of Biology | ||
| Volume 42, Issue 1, March 2019, Pages 43-51 PDF (681.72 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2019.463334 | ||
| Authors | ||
| Maha M. Ashour* ; Ahmed Abd Elrazak; Mohammad I. Abdel-Hamid | ||
| Botany Department, Faculty of Science, University of Mansoura, Egypt | ||
| Abstract | ||
| Nine marine microalgae species namely (Pseudanabaena sp, Chlorella sorokiniana, Chlorella salina, Dunaliella salina, Amphora marina and Phaeodactylum tricornutum were selected to test their potentialities for production of polyunsaturated fatty acids (PUFAs). The screening strategy for the production of PUFAs in this study was GC/MS analysis. The results indicated that diatoms followed by green microalgae were the potential producers of PUFAs. Among the tested producers, Phaeodactylum tricornutum found to be the hightest potential alga to produce PUFA relatively high levels of EPA in particular. A Plackett–Burman statistical design of experiments was applied to screen the effect of different factors including Ca(NO3)2.4H2O, K2HPO4.3H2O, MgSO4.7H2O, Na2SiO3.9H2O, Na2CO3, H3BO3, MnCl2.4H2O, CuSO4.5H2O, HMoO4, ZnCl2, CoCl2.6H2O, FeCl3.6H2O, Na2EDTA.2H2O, Spirulina filtrate, Na2SeO3.5H2O, AlCl3 and glycerol as components of a production medium. This optimization strategy led to a significant increase in the amount of EPA produced by Phaeodactylum tricornutum, where the amount of EPA increased from 1.6 mg/g biomass to 14.5 mg/g. | ||
| Keywords | ||
| Diatoms; eicosapentaenoic acid (EPA); Plackett–Burman; Phaeodactylum tricornutum; polyunsaturated fatty acids | ||
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