Evaluation of Antibiofilm Activity of Daphnetin (Coumarin derivative) against Pseudomonas Aeruginosa | ||
| Biological and Biomedical Journal | ||
| Volume 3, Issue 2, July 2025, Pages 254-263 PDF (403.8 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bbj.2025.399470.1115 | ||
| Authors | ||
| Amira E. Eid1; Lobna M Abo Elnasr2; Eman E. Elmohamady* 3 | ||
| 1Medical Microbiology, Immunology Department, Faculty of Medicine, Tanta University, Egypt | ||
| 2Anesthesia and Surgical Intensive Care Department, Faculty of Medicine, Tanta University, Egypt | ||
| 3Medical Pharmacology Department, Faculty of Medicine, Tanta University, Egypt | ||
| Abstract | ||
| Nowadays, most P.aeruginosa isolates are multidrug resistant (MDR), so the need for a new drug intervention is increasing. This study aimed to investigate the effect of a natural coumarin derivative, Daphnetin, against P.aeruginosa, particularly its biofilm. Also, the incidence of biofilm formation and the antibiotic susceptibility pattern among isolates were detected. A cross sectional study was conducted in intensive care unit (ICU) involving fifty patients that showed signs of infection and after taking a proper history for the participants the samples taken underwent the following: Sample culture, identification of isolates by colony morphology, Gram stain, biochemical tests, antibiotic susceptibility testing by Kirby-Bauer method then detection of biofilm formation by Micro titer plate assay (MPA) after that evaluation of the effect of Daphnetin as following: preparation of Daphentin (DAP) stock solution, making serial dilutions of DAP (0.055-1.781mg/ml), assessing the bacterial growth treated with these different concentrations using optical density (OD600). The results showed that P.aeruginosa was the most prevalent pathogen common in burn swabs (42.9%), followed by endotracheal aspirates (28.5%). Most isolates were biofilm producers (71.4%), 28.6% of isolates were sensitive to amikacin, ciprofloxacin, meropenem, and imipenem, and 42.9% of isolates were sensitive to piperacillin, tazobactam, and ceftazidime. At the effective DAP dosage (0.445–1.781 mg/mL), the amount of biofilm progressively declined. It was concluded that P.aeruginosa is an important opportunistic pathogen. Management of biofilm-forming P.aeruginosa is more difficult. DAP plays a consequential role in the inhibition of biofilm formation | ||
| Keywords | ||
| Antibiotics; Biofilm; Daphnetin; Multi-drug resistance Pseudomonas aeruginosa | ||
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