Isolation and Molecular Diagnosis of Bacteria from the Contact Lenses of Women | ||
| Journal of Bioscience and Applied Research | ||
| Articles in Press, Accepted Manuscript, Available Online from 03 November 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jbaar.2025.374691.1183 | ||
| Authors | ||
| AMJAD IBRAHIM Oraibi* 1; Mohammed Al-SAEDI2; Mohammed jabbar3; rusul Jabbar4; ali adnan5 | ||
| 1Almanara college for medical sciences | ||
| 2Misan Health Directorate, Department of forensic medicine | ||
| 3Misan Health Directorate, Department of Forensic Medicine | ||
| 4Al-Manara College for Medical Sciences, Misan, Iraq ,62001 | ||
| 5Al-Manara College for Medical Sciences, Misan, Iraq ,62001 and Misan Health Directorate, AL-sader Teaching Hospital , Misan, Iraq | ||
| Abstract | ||
| This study was conducted to isolate and identify the bacterial species responsible for causing eye inflammation among individuals who use contact lenses. A total of 25 samples were collected from the contact lens storage fluid of participants, and laboratory diagnostic methods were used to detect the presence of microbial contamination. Out of the total samples, 15 showed bacterial growth. The most commonly isolated bacteria were Staphylococcus aureus (Gram-positive) and Pseudomonas spp. (Gram-negative), each accounting for 33% of the positive cases. In addition, Streptococcus pneumoniae and Staphylococcus epidermidis, both Gram-positive, were detected in 13% of the samples, while Escherichia coli, a Gram-negative bacterium, appeared in 6.6% of the cases. The antibiotic sensitivity test revealed that Cefazolin and Cefalexin were highly effective against all bacterial isolates. In contrast, Penicillin and Amoxicillin showed complete resistance. The results emphasize the importance of lens hygiene and suggest appropriate antibiotic choices for treating related eye infections. | ||
| Keywords | ||
| contact lenses; E. coli; Gram stain; antibiotics; and samples | ||
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