Characterization and synthesis of drug deliver polymers for loading Spirulina using chitosan nanoparticles | ||
| Assiut Veterinary Medical Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 03 November 2025 | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.2025.396237.1769 | ||
| Authors | ||
| Rasha Emad Ahmed AL-Ghazaly1; Hawraa H. Naji1; Adnan M. Jassim* 2; Essa Daham Alhtheal3 | ||
| 1College of Veterinary Medicine, AL-Qasim green University, Babylon 51001, IRAQ. | ||
| 2College of Veterinary Medicine, AL-Qasim green University, Babylon 5013,IRAQ. | ||
| 3Nanotechnology and Advanced Materials Research Center, University of Technology, Baghdad, Iraq | ||
| Abstract | ||
| Chitosan, a chitin biopolymer, is earning academic interest because of its unique properties and applications. Spirulina-loaded chitosan enhances antioxidant, antimicrobial, and wound healing properties, offering a natural, biocompatible system for drug delivery, tissue engineering, and skincare applications with improved bioactivity and sustainability. Preparation of nanoparticles for loading of the materials (ion gelation methods). Loading of Spirulina on Chitosan Nanoparticles (SCNPs), to prepare SCNPs (200 mg) of spirulina powder was dissolved in 5 ml distilled water and gradually added to chitosan solution under stirring at 900 rpm for 30 minutes). The mixture underwent 1-minute sonication, followed by the addition TPP (0.25%) in a 5:1 ratio. After further stirring and sonication, the solution was filtered and centrifuged at 10,000 rpm for 15 minutes. The resulting pellet (SCNPs) was collected and stored at 4°C. The obtained nanoparticles were characterized using different technique (particle size, UV, zeta potential, X-ray diffraction, FTIR (Fourier transform infrared spectroscopy) analysis, Field Emission Scanning Electron Microscopy and hemolytic activity). The results of the current study showed that the use of chitosan alone or after using vitamin ETPGS as emulsifier that recorded played a role in reducing the nanoparticle size to 93.2 nm compared to chitosan alone. It also enhanced the stability of the nanoparticles by increasing their dispersibility, improving the zeta potential, and producing nanoparticles that were uniform and oval in shape. On the other hand, the spirulina-based nanoparticles showed no adverse effects on blood and did not cause any undesirable effects or complications. | ||
| Keywords | ||
| Chitosan; Nanotechnology; FSEM; zeta potential; spirulina | ||
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