Possible Role of Autophagy in Monosodium Glutamate-induced Bladder Overactivity in Adult Male Rats | ||
| Journal of Current Medical Research and Practice | ||
| Volume 10, Issue 4, October 2025, Pages 263-270 PDF (494.44 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jcmrp.2025.406283.1325 | ||
| Authors | ||
| Heba M. Iraqy; Mennat Allah A.M. Ahmed* ; Eman S.H. Abd Allah; Marwa A. Ahmed | ||
| Medical Physiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt. | ||
| Abstract | ||
| Abstract: Introduction Autophagy is an intracellular recycling process that protects cells against various cellular stressors, including oxidative stress, through autophagosome formation and degradation. LC3-II and Beclin-1 are regarded as the key proteins responsible for autophagosome formation. Overactive bladder (OAB) is a bladder disorder characterized by increased sensitivity of the detrusor muscle, leading to urgency, and a common contributor underpinning the pathophysiology of the condition is increased production of local ROS. Hence, we investigated the role of autophagy as a possible mechanism for improving machinery in the MSG MSG-induced OAB rat model. Patients and Methods Two groups of adult male Wister albino rats (n=10) were kept for 4 weeks and randomly designated into a control group that received the vehicle, 0.9% saline orally, and a monosodium glutamate (MSG) group that received saline dissolved monosodium glutamate orally for induction of bladder overactivity. Following sacrifice, assessment of 10-minute induced acetylcholine (ACh) detrusor muscle contractile activity of the study groups in vitro was recorded. Measurement of malondialdehyde (MDA) level in bladder tissue homogenate was conducted by ELIZA; meanwhile, gene expression of detrusor muscle LC3-II and Beclin-1 was measured by qPCR. Results A significant increase in the 10-minute ACh-induced bladder contraction of the MSG group was associated with significantly higher MDA levels and upregulation of LC3-II and Beclin-1 mRNA expression compared to the control group. Conclusion Autophagic machinery is highly activated in OAB conditions due to increased local ROS production, but unfortunately, it didn't improve the condition. Abbreviations OAB; Overactive bladder, MSG; monosodium glutamate, ROS; Reactive oxygen species, MDA; malondialdehyde, LC3-II; microtubule-associated protein-light chain 3, qPCR; quantitative polymerase chain reaction, ULK; Unc-51-like kinase, Atg; Autophagy-related protein, FIP200; the focal adhesion kinase family interacting protein of 200 kD, Vps34; Vacuolar protein sorting 34, PI3K CIII; Phosphatidylinositol 3-Kinase class III. | ||
| Keywords | ||
| Keywords Overactive bladder; monosodium glutamate; Beclin-1; MDA | ||
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