Determination the Antimicrobial Activity of Copper Oxide Synthesized by Extracellular Lactobacillus Plantarum against some Multidrug Resistant Bacteria Isolated from Urinary Tract Infection | ||
| Egyptian Journal of Medical Microbiology | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 July 2026 PDF (759.73 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.435764.1958 | ||
| Authors | ||
| Rehab S. Kurdy* 1; Mohammed N. Maaroof2; Laith A. Yaaqoob3; Safa L. Saleh4 | ||
| 1Department of Biology, College of Education for Pure Sciences,Tikrit university, Tikrit, Iraq | ||
| 2Department of Biology, College of Sciences, Tikrit University, Tikrit, Iraq | ||
| 3Department of Biotechnology, College of Science, University of Baghdad | ||
| 4Department of Biology, College of Education for Pure Sciences,Tikrit University, Tikrit, Iraq | ||
| Abstract | ||
| Background: Copper oxide nanoparticles (CuO NPs) have attracted increasing attention for their versatile applications in catalysis, biosensing, anticancer therapy, and other biomedical fields. Green synthesis using microbial extracellular components offers a sustainable and eco-friendly alternative to conventional chemical methods. Objective: This study aimed to elucidate the biosynthetic process of copper oxide nanoparticles (Cu(NO₃)₂ NPs) utilizing extracellular components from the environmental isolate Lactobacillus plantarum as a natural reducing and stabilizing agent. Methodology: For nanoparticle synthesis, 1 g of copper nitrate was added to 10 mL of extracellular filtrate. The obtained CuO NPs were characterized using Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FTIR), and Field Emission Scanning Electron Microscopy (FE-SEM). Antibiotic resistance profiles of the bacterial isolates and the antibacterial activity of the CuO NPs were assessed. Results: FE-SEM analysis revealed spherical CuO nanoparticles forming nano-cluster aggregates. All isolates exhibited 100 % resistance to cefotaxime and 91 % resistance to ceftriaxone and lincomycin, while resistance to other antibiotics ranged from 18 % to 73 %. Multidrug resistance was observed in all isolates, with at least four antibiotics resisted. The biosynthesized CuO NPs showed strong antibacterial activity, producing inhibition zones of 33 mm against Staphylococcus saprophyticus (150 % concentration) and 19 mm against Burkholderia cepacia (100 % concentration). Conclusion: Lactobacillus plantarum proved effective for the eco-friendly biosynthesis of CuO NPs. Given the high prevalence of antibiotic resistance, these nanoparticles demonstrate promising potential as alternative antimicrobial agents against multidrug-resistant pathogens. | ||
| Keywords | ||
| CuO NPs; biosynthesis nanoparticles; Extracellular; Pathogenic bacterial; Multidrug resistant | ||
|
Statistics Article View: 22 PDF Download: 7 |
||
