Assessment of Hepatotoxicity Induced by Silver Nanoparticles and Possible Therapeutic Effect of Silymarin and Vitamin E in Adult Male Albino Rat: Histological, immunohistochemical and Biochemical study | ||
| Fayoum University Medical Journal | ||
| Volume 16, Issue 1, November 2025, Pages 10-30 PDF (3.79 M) | ||
| Document Type: Full Length research Papers | ||
| DOI: 10.21608/fumj.2025.352482.1444 | ||
| Authors | ||
| Asmaa Wageh Tahif Tawfik1; Abdullah Mahmoud Samy2; Maha Khaled Abdel Wahed3; shimaa aly rouby* 4 | ||
| 1Anatomy & Embryology, Fayoum University,Fayoum ,Egypt | ||
| 2Anatomy department | ||
| 3Anatomy & Embryology, Fayoum University,Fayoum, Egypt | ||
| 4Anatomy and Embryology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt. | ||
| Abstract | ||
| Introduction: Silver Nanoparticles (AgNPs) have hepatotoxic effects which may be secondary to oxidative stress enhancement. The antioxidants Silymarin and Vitamin E can counteract such effects. Aim of the study: to clarify the histopathological, immunological and bio-chemical changes that occur in liver following AgNPs administration and the possible therapeutic action of Silymarin and Vitamin E supplementation. Subjects and Methods: sixty adult male albino rats were divided into: GІ(Control):received no drugs. GII(Sham control): subdivided into subgroup II-A: received normal saline orally for 30 days and subgroup II-B: received 4ml/kg/d olive oil orally for 30 days. GIII(AgNPs): received 0.5mg/kg/day AgNPs I.P. for 30 days. GIV(Silymarin): injected AgNPs for 30 days, then starting from the 31st day, was given oral Silymarin (100 mg/kg /d) for 30 ds. GV(Vitamin E): injected AgNPs for 30 ds, then, starting from the 31st day, 400mg/kg/day vitamin E was given orally for 30 days. GVI (Silymarin and Vitamin E): injected AgNPs for 30 days, then, starting from the 31st day, silymarin and vitamin E was giving orally concomitantly for 30 days. All specimens were prepared for histological, immunohistochemical and biochemical examinations. Results: GIII showed variable pathological changes such as portal, central veins and sinusoids dilatation and congestion, hemorrhage, mononuclear cellular infiltration, and hepatocyte degenerative alterations. Silymarin and vitamin E treated groups displayed relieve of the pathological changes, which were most evident with combined therapy in GVI. Conclusions: Co-antioxidant therapy exerted the most efficient protective effects against AgNPs induced hepatotoxicity. Keywords: Liver; Silver Nanoparticles; Silymarin; Vitamin E; BCL-2. | ||
| Keywords | ||
| Liver; Silver Nanoparticles; Silymarin; Vitamin E; BCL-2 | ||
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