Role of microRNA-223 and High Mobility Group box 1 as Predictors of Drug-resistant Epilepsy | ||
| Egyptian Journal of Medical Microbiology | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 July 2026 PDF (686.45 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.433913.1945 | ||
| Authors | ||
| Randa A. El Zohne1; Osama B. Seddik1; Wafaa M. A. Farghaly2; Mohamed M. Shehab2; Safaa A. Samir1; Menna Al-Adl* 3; Yousra M. Mammdoh1 | ||
| 1Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt | ||
| 2Department of Neurology and Psychiatry, Faculty of Medicine, Assiut University, Assiut, Egypt | ||
| 3Biochemistry Division, Chemistry Department, Faculty of Science, Mansoura University | ||
| Abstract | ||
| Background: Epilepsy is a prevalent neurological illness, impacting about 70 million individuals around the world. Although a large number of patients are controlled with anti-seizure drugs (ASDs), 30%–40% of patients fail to be controlled and develop drug-resistant epilepsy (DRE). Objectives: This study aimed to identify miRNa-223 expression and serum level of high mobility group box 1 (HMGB1) as biomarkers for detecting DRE. Methodology: This case-control study comprised 96 subjects categorized to three groups: group I: 46 patients with genetically presumed epilepsy who having DRE; group II: 25 patients with medically controlled genetically presumed epilepsy and group III: 25 healthy individuals. MiRNA-223 expression level was measured by real-time PCR and serum HMGB1 was estimated by ELISA technique. Results: MiRNA-223 expression serum HMGB1 and hs-CRP levels in epilepsy patients were considerably greater than in patients with controlled epilepsy and in healthy controls (p<0.001 for all). The predictive ability of miRNA, HMGB1 and hs-CRP for the detection of epilepsy and DRE using the ROC curve analysis revealed good sensitivities and specificities. Conclusion: MiRNA-223 expression and serum HMGB1 and hs-CRP levels are important biomarkers for diagnosis of epilepsy in suspicious cases. They are also significant for predicting DRE which may pave the way to the development of new antiepileptogenic drugs. | ||
| Keywords | ||
| Epilepsy; Anti-Seizure Drugs-; Drug-Resistant Epilepsy; MicroRNA-223 -; HMGB1 Protein | ||
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