Age Related Changes in the Skin of Female Albino Rats: Histological, Immuno-histochemical and Biochemical Study | ||
| Egyptian Journal of Histology | ||
| Articles in Press, Accepted Manuscript, Available Online from 10 November 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2025.380529.2269 | ||
| Authors | ||
| Mohamed Zakaria Kotb* 1; Ebthal Ali Ahmed2; Doaa Mahmoud Shuaib3; Al-Moatasem Bellah Al-Sherif4; Mai Mohamed El-Nahla5 | ||
| 1Lecturer, Anatomy Department, Faculty of Medicine, Cairo University | ||
| 2demonstrator, Anatomy Department, Faculty of Medicine, Cairo University | ||
| 3assistant professor, Anatomy department, faculty of medicine Cairo university. | ||
| 4Professor, Anatomy Department, Faculty of Medicine, Cairo University | ||
| 5Lecturer of Anatomy and Embryology, Kasr Alainy ,Cairo University | ||
| Abstract | ||
| The epidermis, dermis, and hypodermis are the main layers that form the skin. With aging, the skin undergoes structural and biochemical changes influenced by both intrinsic genetic factors and extrinsic environmental stressors. Understanding these age-related changes is vital for advancing dermatological and gerontological research. The current study sought to illustrate the histological and biochemical alterations associated with aging skin in the female albino rat. Four equal groups of twenty adult male albino rats were established (weaning, prepubertal, adolescent, and reproductive senescence groups). Histological examination was done using hematoxylin and eosin, orcein, and Masson's trichrome stains. Proliferating cell nuclear antigen (PCNA) immunohistochemical staining was performed. Measurements of serum glutathione reductase enzyme (GR) were also performed. Progressive aging is associated with notable histological changes. The epidermis and dermis were thickened from weaning through adolescence, followed by marked thinning in the reproductive senescence group. Collagen fibers increased in adolescence but declined significantly in the reproductive senescence group. The elastic fibers showed an initial increase in the weaning and prepubertal groups, then a decrease in the adolescent and reproductive senescence groups. The hair follicles and sebaceous glands showed a significant decrease with advancement of age. PCNA immunoreactivity decreased with age, indicating reduced cellular proliferation. GR activity peaked in the prepubertal stage, declined in adolescence, and showed a partial rebound in senescence. These findings reflect the multifactorial nature of skin aging and offer valuable insights into age-related dermatological changes, potentially informing anti-aging strategies and therapeutic interventions. Our study concluded that with advancement of age, the epidermis became thinner and flattened, hair follicles and sebaceous glands atrophied, and the skin thickness reduced. | ||
| Keywords | ||
| histological; biochemical; skin; aging; rats | ||
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