Effect of In-ovo Bee Venom Injection on Hatchability, Blood Traits, Immunity, and Antioxidant Status of Sinai chick’s Strain at Hatch | ||
| Damietta Journal of Agricultural Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 11 November 2025 PDF (1.2 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/djas.2025.465330 | ||
| Authors | ||
| Sara Hassab1; Yaser Rizk2; Mona Elsharawy1; Khaled Elkholy* 1 | ||
| 1Animal, Poultry and Fish Production Department, Faculty of Agriculture, Damietta University, Egypt | ||
| 2Anim. Prod. Res. Instit., Agric. Res. Center, Minis. of Agric. Dokki, Giza, Egypt | ||
| Abstract | ||
| This study was conducted to evaluate the effect of in-ovo bee venom (BV) on hatchability, blood traits, immunity, and antioxidant status of Sinai chickens. A total of 200 eggs were divided into five groups: non-injected control (CON), saline-injected control (sham), and BV-injected groups (10, 15, and 20 µg/egg). On day 18 of incubation, the injections were administered into the air sac. Results indicated that in-ovo injection of bee venom improved the hatchability of Sinai strain eggs, particularly at 10 and 15 µg, and enhanced blood biochemistry by increasing glucose, total protein, albumin, and HDL-C while reducing cholesterol, triglycerides, total lipids, hemoglobin, and MCHC. At 15 µg, ALT decreased and T4 increased, while both 15 and 20 µg raised T3, boosted immunity (WBC, IgG, IgM), and improved antioxidant status by elevating TAC and lowering MDA. In conclusion, in-ovo injection of 15 and 20 µg BV/egg improved immunity, antioxidant status, and hematological traits of Sinai chicks, which may enhance their future performance | ||
| Keywords | ||
| Antioxidant, bee venom; in-ovo; hatchability; Sinai chickens | ||
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