Probiotics as Alternative Antimicrobial Agents Against Enteric Bacteria from Diarrheal Patients | ||
| Minia Journal of Medical Research | ||
| Articles in Press, Accepted Manuscript, Available Online from 12 November 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjmr.2025.433934.2113 | ||
| Authors | ||
| yara sayed mohamed1; Wedad Mahmoud abdelreheam2; mahmoud shokry mahmoud1; Safaa Said Hammad* 3 | ||
| 1microbiology and immunology department faculty of medicine minia university | ||
| 2Minia ,Eygpt | ||
| 3Department of microbiology and immunology ,faculty of medicine, Minia university ,Minia , Egypt | ||
| Abstract | ||
| Background: Probiotic strains are beneficial microorganisms that contribute to maintaining the balance of intestinal microflora and overall gut health. Their antimicrobial effect plays a crucial role in inhibiting the growth of intestinal pathogenic microbes. Objectives: The goal of this study is to isolate and describe probiotics from milk and dairy products and analyze their antimicrobial effect against stool specimens from diarrheic children. Methods: a cross-sectional laboratory study involving 36 children under 5 with acute diarrhea and 6 local market fermented dairy products were conducted. Isolated stool samples were cultured in selective media to identify pathogens . Dairy samples were enhanced to isolate probiotics. Antimicrobial activity was assessed by the agar well-diffusion assay Results: Four types of probiotics were identified. All isolates were Gram-positive, non-motile, indole-negative and citrate-negative. Among the isolates, probiotics 1 and probiotics 2 demonstrated the greatest antimicrobial activity, averaging inhibition zones of nearly 12 mm against E. coli and K. pneumoniae. Conclusion: Probiotic strains exhibiting antimicrobial activities can be found in locally produced dairy products. Most effective against enteric pathogens was probiotics 1 and probiotics 2, suggesting their value as biotherapeutic agents for pediatric diarrhea. Further molecular work and in vivo studies are needed. | ||
| Keywords | ||
| Keywords Probiotics; Lactic acid bacteria; Fermented dairy products; Antimicrobial activity | ||
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