Production of proteolytic enzymes from a newly isolated Bacillus subtilis strain via submerged fermentation for agricultural applications | ||
| Journal of Environmental Studies | ||
| Articles in Press, Accepted Manuscript, Available Online from 13 November 2025 | ||
| Document Type: High quality original papers | ||
| DOI: 10.21608/jesj.2025.413330.1147 | ||
| Authors | ||
| Erdal Alsancak1; Burcu Atlı* 2; İdil Dayankaç Öztürk1; Havva Betül İstanbullu Çobanoğlu1; Gökçe Ulaş1 | ||
| 1Hektas High Technology Center | ||
| 2Hektas Ankara High Technology Center | ||
| Abstract | ||
| This study aimed to evaluate the extracellular protease production potential of Bacillus isolates obtained from different soil samples from various regions of Türkiye. The overarching goal was to identify a high-yielding local isolate that could be further developed for agricultural applications, particularly in soil remediation. Eighty-six Bacillus isolates (B1–B86) were screened for extracellular protease activity using casein-milk agar plates. The five most active isolates were selected for further analysis based on the size of the inhibition zones. Among these, isolate B64 was identified as Bacillus subtilis using the MALDI-TOF identification method. The proteolytic activities of B64 were optimized under submerged fermentation conditions in a 5 L lab-scale bioreactor. SDS-PAGE analysis was used to determine the molecular weight of the crude enzyme. Isolate B64, identified as Bacillus subtilis, demonstrated the highest protease (9.8 U/mL) and milk clotting (738 SU/mL) activities, under the specified conditions. Molecular weight of the crude protease enzyme was approximately 44 kDa. The crude protease from Bacillus subtilis B64 isolate exhibits promising properties for potential use in agricultural applications, especially in soil remediation processes. | ||
| Keywords | ||
| Bacillus species; proteolytic enzymes; MALDI-TOF; fermentation; sustainable agriculture | ||
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