Modulatory effects of Visfatin and Resistin on host immunity during Entamoeba histolytica infection | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 14 November 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.436039.3378 | ||
| Authors | ||
| Ahmed Wisam Azeez1; Asraa Dawod Farhan2; Mohammed Abdul Ameer Shawkat2; Osama A mohsein* 3 | ||
| 1Department of Biology, Faculty of Education for Pure Science, University of Diyala, Diyala, Iraq | ||
| 2Department of Biology, College of Sciences, University of Diyala, Diyala, Iraq | ||
| 3Department of Medical Laboratory Techniques, Mazaya University College, Thi-Qar, Iraq | ||
| Abstract | ||
| Background: Visfatin and resistin, two key adipokines, have significant effects on inflammatory and immune pathways and, therefore, determine host defense mechanisms during Entamoeba histolytica infection. Objectives: This research will seek to examine the effect of visfatin and resistin in regulating the immune response in E. histolytica-infected patients and to determine their relationships with the biomarkers of inflammatory and oxidative stress. Methods: The case-control study was performed between March 20 and July 10, 2025, at Nasiriyah General and Al-Habboubi Teaching hospitals, Iraq whereby 100 patients of E. histolytica and 50 healthy controls took part in it. The adults who had diarrhea and positive stool and antigen ELISA test were taken into account, but not those who had other infections, autoimmune, and chronic inflammatory diseases. ELISA, stool microscopy, proved infected. Blood samples were taken, serum separated and processed at -80 o C. ELISA and standard biochemical measurements of Visfatin, Resistin, CRP, TNF- a, IL- 6, MDA, SOD, and GPx were carried out biochemical methods. Results: The study did not find any significant difference in age or gender between patients and controls, but patients had diarrhea that was significantly more common (78%, p < 0.001). There were significant increases in immune biomarkers with Visfatin (12.50 ± 3.20 vs. 8.10 ± 2.50 ng/mL) and Resistin (18.30 ± 4.10 vs. 11.70 ± 3.60 ng/mL) being significantly increased (p < 0.001). There was also a significant increment of inflammatory markers CRP, TNF-a, and IL-6, oxidative stress marker MDA and antioxidant enzymes SOD and GPx improved, which showed significant immune activity and oxidative imbalance in E. histolytica infection. Conclusions: The E, histolytica infection is associated with a high level of Visfatin, Resistin, and pro-inflammatory factors and a low level of antioxidant protection as evidence of immune activation and oxidative stress. This indicates that these biomarkers are important in the regulation of host immune response and the progression of the disease. | ||
| Keywords | ||
| Visfatin; resistin; Entamoeba histolytica; Immune response; inflammatory markers | ||
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