Molecular characterization and antimicrobial resistance profiles of Salmonella species from disease outbreaks in commercial poultry farms in North-West Nigeria | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 15 November 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.368664.2632 | ||
| Authors | ||
| Saidu Yakubu* 1; Waziri Ibrahim MUSA2; Lawal SAIDU3; Kabiru Haliru AHMAD4; Paul Habila Mamman5; Aminu Isah MUSA6; Mannir Dahiru Usman7; Tauheed Abubakar Muazu8; Mohammed Shaibu Atabo9; Abdullahi Muhammad10 | ||
| 1College of Agriculture and Animal science Bakura Zamfara State. | ||
| 2Department of veterinary Medicine Faculty of veterinary medicine Ahmadu University Zarian | ||
| 3Veterinary Teaching Hospital, Faculty of veterinary medicine Ahmadu University Zaria | ||
| 4Department of veterinary microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria Nigeria | ||
| 5Department of veterinary microbiology, Faculty of Veterinary Medicine Ahmadu Bello University, Zaria, Nigeria | ||
| 6Africa Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University zaria Kaduna state | ||
| 7Department of Veterinary Medicine, Faculty of Veterinary Medicine, Bayero University Kano, Nigeria | ||
| 8Department of veterinary Anatomy, Faculty of Veterinary Medicine, Bayero University Kano, Nigeria | ||
| 9Department of Basic Sciences School of Veterinary Medicine St. Matthew’s University Grand Cayman, Cayman Islands | ||
| 10Department of veterinary Medicine Faculty of veterinary medicine Ahmadu Bello University Zaria, Nigeria | ||
| Abstract | ||
| Background: Salmonella species are the major bacterial pathogens responsible for disease outbreaks in commercial poultry farms, leading to significant economic losses and public health risks. Aim: To identify Salmonella species associated with disease outbreaks in commercial poultry and evaluate their antimicrobial resistance profiles. Methodology: Samples were collected from farms with a clinical diagnosis of fowl typhoid. Of the 809 samples collected, 144 isolates were identified based on biochemical tests. All the isolates were subjected to antimicrobial sensitivity testing, and nine isolates (three from each state) were identified using polymerase chain reaction (PCR). Data was analysed by descriptive statistics and expressed using simple percentages. Results: PCR confirmed Salmonella spp. in all nine isolates via detection of the invA gene (284 bp). S. gallinarum and S. enteritidis were identified using the flc (571 bp) and sef (535 bp) genes, respectively. The isolates showed high sensitivity to chloramphenicol, ceftriaxone, gentamicin, and ampicillin, but resistance to ciprofloxacin and amoxicillin. Multidrug resistance was observed in 17.5% of isolates, with MAR indices of 0.50 (48%), 0.67 (8%), 0.83 (20%), and 1.00 (24%), indicating significant antibiotic exposure. Conclusion: The study identified S. gallinarum and S. enteritidis as the primary serovars in the outbreaks. The high MAR indices highlight the urgent need for improved antibiotic stewardship in poultry farms in the study areas. | ||
| Keywords | ||
| Salmonellosis; Poultry; Northwest-Nigeria; Epidemiology | ||
|
Statistics Article View: 3 |
||
