Comparative Study on Different Diagnostic Methods of Bovine Tuberculosis in Cattle | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 16 November 2025 PDF (1.12 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.422813.3120 | ||
| Authors | ||
| Shohanda Moustafa Kamel1; Soliman Mohamed Soliman2; attia Abd Allah El Gedawy3; SAMIA ABDEL HAMID AHMED* 4 | ||
| 1Bacteriology department, Animal Health Research Institute. | ||
| 2Department of Internal medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, , Egypt | ||
| 3Bacteriology department, Animal Health Research Institute, Dokki, Giza | ||
| 4Department of Internal medicine and Infectious Diseases, Faculty of Veterinary Medicine, CairoUniversity, , Egypt | ||
| Abstract | ||
| A total of 400 cattle were examined in private farms in El-Sharkia, El-Gharbia and Damietta governorates in accordance with the national control program against bovine tuberculosis. This study aimed to evaluate and compare the diagnostic tests performance for bovine tuberculosis in cattle to determine the most reliable approach particularly under practical field.8.5% of the total examined cattle was positive reactors for single intradermal test, post mortal findings of lymph nodes and internal organs tested for, Conventional PCR using mpb70 gene, culture techniques and Real time Polymerase chain Reaction (PCR). The results were 100%, 82%, 100% respectively. Blood samples were withdrawn for Gamma interferon test and ELISA tests, 54 animals out of 400 were positive by gamma interferon, the positive results were recorded as 21out of 120 (17.5%) in El-Gharbia,15 0ut of 140 (11%) in El-Sharkia while 13% in Damietta. while 100 out of 400 animals were positive for ELISA test. Among the various diagnostic tests for bovine tuberculosis, PCR is regarded as a reliable, rapid and accurate test for bovine tuberculosis diagnosis, particularly in detecting subclinical infections. The phylogenic tree of the sequenced 3 isolates representing the 3governorates shows a close similarity. Consequently, integrating diagnostic techniques improves the detection of bovine tuberculosis. | ||
| Keywords | ||
| Bovine tuberculosis; Single intradermal test; Mycobacterium bovis and Gamma interferon | ||
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