Genetic diversity analysis in barley by ISSR and SCoT markers | ||
| Egyptian Journal of Agronomy | ||
| Volume 47, Issue 4, December 2025, Pages 1315-1325 PDF (717.25 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/agro.2025.372784.1656 | ||
| Authors | ||
| walaa Abdel-kader Ramadan1; Mahmoud Hussien Abou-Deif* 2; Fatma Elsayed Mahmoud3; Rashad Mahmoud Shoaib4 | ||
| 1Genetics and Cytology Department, Biotechnology Institute, National Research Centre, Egypt | ||
| 233 Tahrir St., 12622 Dokki, Giza, Egypt | ||
| 3National Research Centre, Genetics and Cytology Dept., Tahrir St., 12622 Dokki, Cairo, Egypt. | ||
| 4Genetic and Cytology department, Biotechnology Research institute, National Research Center, 12622 Dokki, Giza, Egypt | ||
| Abstract | ||
| Estimating genetic diversity is crucial for managing genetic resources, protecting species in their natural environment, and selecting suitable parental combinations, all of which impact the genomic improvement of crop species. This study aimed to estimate the genetic diversity and relationships among ten Egyptian barley cultivars using molecular genetic markers, inter-simple sequence repeats (ISSRs), and start codon targeted (SCoT). The amplification results obtained by PCR analysis for the studied cultivars, using 12 ISSR and 10 SCoT primers, identified each of the ten cultivars. The total number of markers observed among the ten barley genotypes, based on ISSR analysis, was 953 bands in 126 loci, of which 76 loci were polymorphic, resulting in a polymorphism rate of 60.31%. The two primers ISSR-1 and ISSR-15 distinguished the cultivars in a unique banding pattern for each. The total number of markers detected among the ten cultivars based on SCoT analysis was 864 bands in 121 loci, of which 72 loci were polymorphic (59.50%). The primer SCoT-34 distinguished the barley cultivars into unique banding patterns. The genetic similarity index and dendrogram tree were performed using ISSR and SCoT amplified fragments for the studied cultivars. The values of similarity exhibited considerable differences between the barley cultivars. The similarity ranged from 72 to 94%. The results indicated that two molecular markers, ISSR and SCoT, are efficient in analyzing the genetic diversity in barley cultivars. | ||
| Keywords | ||
| Barley; Molecular genetic markers; PCR analysis; Similarity index; Dendrogram tree | ||
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