Upregulation of miRNA-630 and downregulation of TRAF6, MD2, NGAL, and KIM1 by bee bread alleviate HFD-induced kidney injury | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Articles in Press, Accepted Manuscript, Available Online from 18 November 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2025.433568.2832 | ||
| Authors | ||
| Amira Kamel1; Marwa Hassan Bakr Mohamed2, 3; Raghda Elsherif* 2; Ghada Ezzat1 | ||
| 1Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, Egypt | ||
| 2Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, Egypt, | ||
| 3Basic medical science department, Badr university, Assiut, Egypt. | ||
| Abstract | ||
| There is a connection between renal impairment and obesity and high-fat diet (HFD) consumption. The items that are naturally derived have received little attention. Assessing bee bread's potential to mitigate HFD-induced kidney impairment was the goal of the current investigation. Three groups (10 rats each) of thirty albino male rats were created: control, HFD, and bee bread. For 14 weeks, the control group was given a regular diet; the HFD group received HFD for 14 weeks; and the bee bread group was enriched with HFD for 10 weeks, followed by another 4 weeks of HFD supplemented with bee bread. Following the experiment, colorimetric techniques were used to estimate the blood glucose level during fasting, urea, creatinine, and lipid panel. Quantitative real-time PCR was used to examine miRNA-630, TRAF6, MD2, NGAL, and KIM-1 gene expression. Ultimately, histopathological analyses and PCNA and CD68 immunohistochemical detection were carried out. Weight of body, fasting blood glucose, urea, creatinine, and lipid profile were all noticeably greater in the HFD group. Additionally, the HFD group showed upregulation of TRAF6, MD2, NGAL, and KIM-1 and downregulation of miRNA-630. The biochemical and histological disruptions brought on by HFD were lessened by bee bread. Therefore, we came to the conclusion that bee bread might be a preventive factor against obesity and renal impairment brought on by HFD. | ||
| Keywords | ||
| High-fat diet (HFD); bee bread; miRNA-630; TRAF6; MD2 | ||
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